KCNE3 is an inhibitory subunit of the Kv4.3 potassium channel

被引：26

作者：

Lundby, Alicia ^{[1
]}

Olesen, Soren-Peter ^{[1
]}

机构：

[1] Univ Copenhagen, Panum Inst, Danish Natl Res Fdn, Ctr Cardiac Arrhythmia,Dept Med Physiol, DK-2200 Copenhagen N, Denmark

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 346卷 / 03期

关键词：

voltage-gated potassium channel; I-to current; inhibitory beta-subunit; MIRP2; Kv4.3; KCND3; KCNE3;

D O I：

10.1016/j.bbrc.2006.06.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mammalian Kv4.3 potassium channel is a fast activating and inactivating K+ channel widely distributed in mammalian tissues. Kv4.3 is the major component of various physiologically important currents ranging from A-type currents in the CNS to the transient outward potassium conductance in the heart (I-to). Here we show that the KCNE3 beta-subunit has a strong inhibitory effect on current conducted by heterologously expressed Kv4.3 channels. KCNE3 reduces the Kv4.3 current amplitude, and it slows down the channel activation and inactivation as well as the recovery from inactivation. KCNE3 also inhibits currents generated by Kv4.3 in complex with the accessory subunit KChIP2. We find the inhibitory effect of KCNE3 to be specific for Kv4.3 within the Kv4 channel family. Kv4.3 has previously been shown to interact with a number of beta-subunits, but none of the described subunit-interactions exert an inhibitory effect on the Kv4.3 current. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：958 / 967

页数：10

共 34 条

[1] Disease-associated mutations in KCNE potassium channel subunits (MiRPs) reveal promiscuous disruption of multiple currents and conservation of mechanism [J].