Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering

被引：73

作者：

Danilevicius, Paulius ^{[1
]}

Rekstyte, Sima ^{[1
]}

Balciunas, Evaldas ^{[2
]}

Kraniauskas, Antanas ^{[3
]}

Jarasiene, Rasa ^{[2
]}

Sirmenis, Raimondas ^{[3
]}

Baltriukiene, Daiva ^{[2
]}

Bukelskiene, Virginija ^{[2
]}

Gadonas, Roaldas ^{[1
]}

Malinauskas, Mangirdas ^{[1
]}

机构：

[1] Vilnius State Univ, Fac Phys, Dept Quantum Elect, Laser Res Ctr, LT-10223 Vilnius, Lithuania

[2] Vilnius State Univ, Inst Biochem, LT-08662 Vilnius, Lithuania

[3] Vilnius State Univ, Hosp Santariskiu Klin, Heart Surg Ctr, LT-08661 Vilnius, Lithuania

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 08期

关键词：

direct laser writing; multi-photon polymerization; 3D scaffolds; biocompatible photopolymers; cardiovascular grafts; stem cell; tissue engineering; 2-PHOTON POLYMERIZATION; STEM-CELLS; ACID); PHOTOPOLYMERIZATION; DIFFERENTIATION; BIOMATERIALS; LITHOGRAPHY; CONSTRUCTS; COPOLYMERS; MONOMERS;

D O I：

10.1117/1.JBO.17.8.081405

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

This work presents the latest results on direct laser writing of polymeric materials for tissue engineering applications. A femtosecond Yb:KGW laser (300 Is, 200 kHz, 515 nm) was used as a light source for non-linear lithography. Fabrication was implemented in various photosensitive polymeric materials, such as: hybrid organic-inorganic sol-gel based on silicon-zirconium oxides, commercial ORMOCER (R) class photoresins. These materials were structured via multi-photon polymerization technique with submicron resolution. Porous three-dimensional scaffolds for artificial tissue engineering were fabricated with constructed system and were up to several millimeters in overall size with 10 to 100 mu m internal pores. Biocompatibility of the used materials was tested in primary rabbit muscle-derived stem cell culture in vitro and using laboratory rats in vivo. This interdisciplinary study suggests that proposed technique and materials are suitable for tissue engineering applications. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.8.081405]

引用

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