4-N,N-Dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-benzofurazan as a new precolumn fluorescence derivatization reagent for carboxylic acids (fatty acids and drugs containing a carboxyl moiety) in liquid chromatography

被引：23

作者：

Prados, P

Fukushima, T

Santa, T

Homma, H

Tsunoda, M

AlKindy, S

Mori, S

Yokosu, H

Imai, K

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,DEPT BIOANALYT CHEM,BUNKYO KU,TOKYO 113,JAPAN

[2] SULTAN QABOOS UNIV,DEPT CHEM,AL KHOUD,OMAN

[3] TOKYO KASEI KOGYO CO LTD,KITA KU,TOKYO 114,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 344卷 / 03期

关键词：

4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED); 4-N,N-dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadiazole (DBD-Cl); liquid chromatography; fatty acid; 2-arylpropionic acid;

D O I：

10.1016/S0003-2670(97)00020-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new fluorescent reagent for carboxylic acids, 4-N,N-dimethylaminosulfonyl-7-N-(2-amino adiazole (DBD-ED) was synthesized from 4-N,N-dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadiazole (DBD-Cl) and 1,2-diaminoethane. DBD-Cl is also a new compound described in this work for the first time. The applicability of DBD-ED as a precolumn derivatization reagent in liquid chromatography (LC) was examined. DBD-ED reacted with saturated fatty acids and non-steroidal anti-inflammatory drugs (NSAIDs) in the presence of triphenylphosphine and 2,2'-dipyridyl disulfide at room temperature for 30 min for NSAIDs and 1 h for fatty acids to give fluorescent products. The reaction solutions were subjected to LC and the derivatized products were detected spectrofluorimetrically at 560 nm with excitation at 450 nm. The detection limits were in the fmol range.

引用

页码：227 / 232

页数：6

共 17 条

[1] 9-DIAZOMETHYLANTHRACENE AS A NEW FLUORESCENCE AND ULTRAVIOLET LABEL FOR THE SPECTROMETRIC DETECTION OF PICOMOLE QUANTITIES OF FATTY-ACIDS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].