Determination of aliphatic amines in water using derivatization with fluorescein isothiocyanate and capillary electrophoresis laser-induced fluorescence detection

被引:30
作者
Brumley, WC
Kelliher, V
机构
[1] U.S. Environmental Protection Agency, Natl. Exposure Research Laboratory, Environmental Sciences Division, Las Vegas, NV 89193-3478
关键词
D O I
10.1080/10826079708006556
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Detection-oriented derivatization of aliphatic amines and amine functional groups in compounds of environmental interest was studied using fluorescein isothiocyanate (FITC) with separation/determination by capillary electrophoresis/laser-induced fluorescence. Determinative levels down to 10 ppb were studied in deionized and environmental waters. Practical detection limits of 30 to 100 ppb or more were realized, depending on analyte and water source. Criteria were developed to compare results to desired performance in a derivatization method. FITC was judged to be a moderately successful reagent with drawbacks including extensive by-products, lack of specificity to analyte class, requirement to study each potential analyte carefully, and derivatization limits near 30 ppb. Advantages include low cost of reagent, ease in handling, and synthesis of an ionized derivative for free zone electrophoresis.
引用
收藏
页码:2193 / 2205
页数:13
相关论文
共 37 条
[1]   USE OF 3-(P-CARBOXYBENZOYL)QUINOLINE-2-CARBOXALDEHYDE TO LABEL AMINO-ACIDS FOR HIGH-SENSITIVITY FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS [J].
ARRIAGA, EA ;
ZHANG, YN ;
DOVICHI, NJ .
ANALYTICA CHIMICA ACTA, 1995, 299 (03) :319-326
[2]   GAS-CHROMATOGRAPHY MASS-SPECTROMETRY DETERMINATION OF WATER-SOLUBLE PRIMARY AMINES AS THEIR PENTAFLUOROBENZALDEHYDE IMINES [J].
AVERY, MJ ;
JUNK, GA .
ANALYTICAL CHEMISTRY, 1985, 57 (04) :790-792
[3]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF SERUM ALIPHATIC-AMINES IN CHRONIC-RENAL-FAILURE [J].
BABA, S ;
WATANABE, Y ;
GEJYO, F ;
ARAKAWA, M .
CLINICA CHIMICA ACTA, 1984, 136 (01) :49-56
[4]   SOLID-PHASE REAGENT CONTAINING THE 3,5-DINITROPHENYL TAG FOR THE IMPROVED DERIVATIZATION OF CHIRAL AND ACHIRAL AMINES, AMINO-ALCOHOLS AND AMINO-ACIDS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION [J].
BOURQUE, AJ ;
KRULL, IS .
JOURNAL OF CHROMATOGRAPHY, 1991, 537 (1-2) :123-152
[5]   ELECTROPHORETIC BEHAVIOR OF AROMATIC-CONTAINING ORGANIC-ACIDS AND THE DETERMINATION OF SELECTED COMPOUNDS IN WATER AND SOIL BY CAPILLARY ELECTROPHORESIS [J].
BRUMLEY, WC ;
BROWNRIGG, CM .
JOURNAL OF CHROMATOGRAPHY, 1993, 646 (02) :377-389
[6]   TECHNIQUES WITH POTENTIAL FOR HANDLING ENVIRONMENTAL-SAMPLES IN CAPILLARY ELECTROPHORESIS [J].
BRUMLEY, WC .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1995, 33 (12) :670-685
[7]  
Brumley WC, 1995, LC GC N AM, V13, P556
[8]  
BRUMLEY WC, 1996, APPL LC MS ENV CHEM, P481
[9]  
COLE WD, 1996, 20 INT S HIGH PERF L
[10]  
DUONG KD, 1991, ANALUSIS, V19, P154