Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation

被引：7

作者：

Asano, K ^{[1
]}

Nakamura, M ^{[1
]}

Oguma, T ^{[1
]}

Fukunaga, K ^{[1
]}

Matsubara, H ^{[1
]}

Shiomi, T ^{[1
]}

Ishizaka, A ^{[1
]}

Yamaguchi, K ^{[1
]}

Kanazawa, M ^{[1
]}

机构：

[1] Keio Univ, Sch Med, Dept Med, Cardiopulm Div,Shinjuku Ku, Tokyo 1608582, Japan

来源：

INFLAMMATION RESEARCH | 2001年 / 50卷 / 12期

关键词：

eosinophil; eotaxin; RANTES; monocyte chemoattractant protein; asthma;

D O I：

10.1007/PL00000244

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Objective and design: The gene expression profile of CCR3 ligands, cotaxin, RANTES, and monocyte chemoattractant protein-3 (MCP-3), was examined in normal and inflamed guinea pig lungs. Material: Male Hartley guinea pigs (n = 49). Methods: Pulmonary mRNA was obtained from naive animals, animals treated with intravenous lipopolysaccharide administration, and animals repeatedly exposed to aerosolized allergen (ovalbumin). Northern analysis was performed to quantify pulmonary expression of eotaxin, RANTES, and MCP-3 mRNA. Pulmonary eosinophil peroxidase (EPO) activity was measured to quantify eosinophil accumulation. Results: Eotaxin and RANTES mRNAs, but not MCP-3 mRNA, were constitutively expressed in guinea pig lungs. Lipopolysaccharide treatment increased MCP-3 mRNA expression, but not eotaxin or RANTES mRNA. In contrast, allergen exposure in sensitized animals caused an increase in eotaxin mRNA, which demonstrated good temporal and quantitative correlation with pulmonary EPO activity, but not in MCP-3 or RANTES mRNA. Conclusions: Guinea pig CCR3 ligands demonstrated different gene expression profiles in normal and inflamed airways, suggesting that they play different physiological and pathophysiological roles in the airway.

引用

页码：625 / 630

页数：6

共 31 条

[1]

Increased MCP-1, RANTES, and MIP-1 alpha in bronchoalveolar lavage fluid of allergic asthmatic patients [J].