Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification

被引：24

作者：

Wakefield, AE

Keely, SP

Stringer, JR

Christensen, CBV

Ahrens, P

Peters, SE

BilleHansen, V

Henriksen, SA

Jorsal, SE

Settnes, OP

机构：

[1] UNIV CINCINNATI,DEPT MOL GENET BIOCHEM & MICROBIOL,CINCINNATI,OH

[2] UNIV COPENHAGEN,PANUM INST,INST MED MICROBIOL & IMMUNOL,DK-2200 COPENHAGEN,DENMARK

[3] DANISH VET LAB,COPENHAGEN,DENMARK

[4] UNIV LONDON QUEEN MARY & WESTFIELD COLL,SCH BIOL SCI,LONDON E1 4NS,ENGLAND

来源：

APMIS | 1997年 / 105卷 / 04期

关键词：

Pneumocystis carinii; pig; DNA amplification; nucleotide sequence; BETA-TUBULIN GENE; INFECT HUMANS; RAT; PNEUMONIA; SEQUENCE; FUNGI; ANTIGENS; STRAINS; SHREWS;

D O I：

10.1111/j.1699-0463.1997.tb00576.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.

引用

页码：317 / 321

页数：5

共 40 条

[1]

ARMSTRONG MYK, 1994, PNEUMOCYSTIS CARINII, P181

[2] PNEUMOCYSTIS-CARINII, AN OPPORTUNIST IN IMMUNOCOMPROMISED PATIENTS [J].