Nocardia sp carboxylic acid reductase:: Cloning, expression, and characterization of a new aldehyde oxidoreductase family

被引：95

作者：

He, AM

Li, T

Daniels, L

Fotheringham, I

Rosazza, JPN ^{[1
]}

机构：

[1] Univ Iowa, Coll Pharm, Div Med & Nat Prod Chem, Iowa City, IA 52242 USA

[2] Univ Iowa, Coll Pharm, Ctr Biocatalysis & Bioproc, Iowa City, IA 52242 USA

[3] Univ Iowa, Coll Med, Dept Microbiol, Iowa City, IA 52242 USA

[4] Schering Plough Corp, Union, NJ 07083 USA

[5] Univ Edinburgh, Dept Chem, Edinburgh, Midlothian, Scotland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 03期

关键词：

D O I：

10.1128/AEM.70.3.1874-1881.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had K-m values for benzoate, ATP, and NADPH that were similar to those for natural CAR, and recombinant CAR reduced benzoic, vanillic, and ferulic acids to their corresponding aldehydes. car is the first example of a new gene family encoding oxidoreductases with remote acyl adenylation and reductase sites.

引用

页码：1874 / 1881

页数：8

共 40 条

[1] Purification and characterization of the coniferyl aldehyde dehydrogenase from Pseudomonas sp. strain HR199 and molecular characterization of the gene [J].