Submillimeter-wave Fourier transform spectroscopy of biological macromolecules

被引:71
作者
Globus, TR [1 ]
Woolard, DL
Samuels, AC
Gelmont, BL
Hesler, J
Crowe, TW
Bykhovskaia, M
机构
[1] Univ Virginia, Dept Elect Engn, Charlottesville, VA 22904 USA
[2] USA, Res Lab, Army Res Off, Res Triangle Pk, NC 27709 USA
[3] USA, Soldier Biol & Chem Command, Aberdeen Proving Ground, MD 21010 USA
[4] Univ Virginia, Charlottesville, VA 22908 USA
关键词
D O I
10.1063/1.1466878
中图分类号
O59 [应用物理学];
学科分类号
摘要
In this article we report experimental results on Fourier-transform infrared spectroscopy of deoxyribonucleic acid (DNA) macromolecules and related biological materials in the submillimeter range (i.e., similar to10-500 cm(-1)). Film samples made from commercial DNA fibers, polyadenylic acid potassium salt, and cellular agents such as the spore form of Bacillus subtillis have been prepared and measured. A broad series of measurements carried out in the low frequency region (10-50 cm(-1)) with a higher resolution of 0.2 cm(-1) revealed fine features-multiple dielectric resonances in the submillimeter-wave spectra obtained from DNA samples. These long-wave absorption features are shown to be intrinsic properties of biological materials determined by phonon modes. The emphasis is on reproducibility of experimental spectra and on receiving reliable results. The effects of differences in sample preparation, including sample geometry, orientation, and aging are studied and separated from the phonon effects that determine the fine structure of transmission spectra. A direct comparison of spectra between different DNA samples reveals a large number of modes and a reasonable level of sequence-specific uniqueness. A theoretical study of two double helical DNA fragments has applied a normal mode analysis to predict spectra in the far infrared. Most of the modes determined by long-distance interactions are at frequencies below 220 cm(-1), with the density higher than one mode per cm(-1), which is approximately what was observed experimentally. (C) 2002 American Institute of Physics.
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页码:6105 / 6113
页数:9
相关论文
共 51 条
[1]  
Bykhovskaia M, 2000, BIOPHYS J, V78, p404A
[2]   Prediction of DNA far-IR absorption spectra based on normal mode analysis [J].
Bykhovskaia, M ;
Gelmont, B ;
Globus, T ;
Woolard, DL ;
Samuels, AC ;
Duong, TH ;
Zakrzewska, K .
THEORETICAL CHEMISTRY ACCOUNTS, 2001, 106 (1-2) :22-27
[3]  
BYKHOVSKAIA M, 2000, ANN M BIOPH SOC NEW
[4]   Effect of drug-binding-induced deformation on the vibrational spectrum of a DNA daunomycin complex [J].
Chen, YZ ;
Szabo, A ;
Schroeter, DF ;
Powell, JW ;
Lee, SA ;
Prohofsky, EW .
PHYSICAL REVIEW E, 1997, 55 (06) :7414-7423
[5]  
Duong TH, 1997, J COMPUT CHEM, V18, P796, DOI 10.1002/(SICI)1096-987X(19970430)18:6<796::AID-JCC5>3.0.CO
[6]  
2-N
[7]   RESONANT MICROWAVE-ABSORPTION OF SELECTED DNA-MOLECULES [J].
EDWARDS, GS ;
DAVIS, CC ;
SAFFER, JD ;
SWICORD, ML .
PHYSICAL REVIEW LETTERS, 1984, 53 (13) :1284-1287
[8]   SOFT MODES AND STRUCTURE OF DNA DOUBLE HELIX [J].
EYSTER, JM ;
PROHOFSKY, EW .
PHYSICAL REVIEW LETTERS, 1977, 38 (07) :371-373
[9]   LATTICE VIBRATIONAL MODES OF POLY(RU) AND POLY(RA) [J].
EYSTER, JM ;
PROHOFSKY, EW .
BIOPOLYMERS, 1974, 13 (12) :2505-2526
[10]   LATTICE VIBRATIONAL MODES OF POLY(RU).POLY(RA) - COUPLED SINGLE-HELICAL APPROACH [J].
EYSTER, JM ;
PROHOFSKY, EW .
BIOPOLYMERS, 1974, 13 (12) :2527-2543