In vitro evaluation of B-CLL cells apoptotic responses to irradiation

被引：8

作者：

Comby, E ^{[1
]}

Andre, I ^{[1
]}

Troussard, X ^{[1
]}

Lebrun, E ^{[1
]}

Sola, B ^{[1
]}

Ballet, JJ ^{[1
]}

Leporrier, M ^{[1
]}

机构：

[1] Univ Caen, UPRES EA2128, F-14000 Caen, France

来源：

LEUKEMIA & LYMPHOMA | 1999年 / 34卷 / 1-2期

关键词：

apoptosis; gamma irradiation; B-CLL; flow cytometry;

D O I：

10.3109/10428199909083392

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Defective apoptosis is a mechanism which could possibly explain B chronic lymphocytic leukemia (B-CLL) cell accumulation. Differences in evolution and prognosis of B-CLL patients may be due to heterogeneity in apoptotic cell death. We studied the apoptotic response to in vitro gamma radiation of blood mononuclear cells from 18 untreated B-CLL patients. In cells irradiated with 2, 4 or 8 Gy and then cultured for 20 hours, the percentage of trypan blue excluding (viable) cells was not modified (>92%). An apoptotic response to irradiation was detected in the majority of the patients, but the individual percentage of apoptotic cells varied widely (8 to 81% after 8 Gy irradiation) in individual cases. The flow cytometric analysis of nick-end DNA labeling demonstrated a dose effect of irradiation, particularly in patients with an apoptotic response of over 20%. In the future, a valuable clue to the selection of irradiation regimens for B-CLL patients may be the investigation of correlations between in vitro radiation-induced apoptosis and the in vivo response to radiation therapy.

引用

页码：159 / 166

页数：8

共 24 条

[1]

AguilarSantelises M, 1996, INT J CANCER, V69, P114, DOI 10.1002/(SICI)1097-0215(19960422)69:2<114::AID-IJC8>3.0.CO

[2]

2-3

[3] ORAL ANTILYMPHOCYTE ACTIVITY AND INDUCTION OF APOPTOSIS BY 2-CHLORO-2'-ARABINO-FLUORO-2'-DEOXYADENOSINE [J].