Allelic loss on chromosome arm 8p: Analysis of sporadic epithelial ovarian tumors

被引:46
作者
Brown, MR
Chuaqui, R
Vocke, CD
Berchuck, A
Middleton, LP
Emmert-Buck, MR
Kohn, EC
机构
[1] NCI, Pathol Lab, Bethesda, MD 20892 USA
[2] NCI, Pathogenet Unit, Pathol Lab, Bethesda, MD 20892 USA
[3] NCI, Urol Oncol Branch, Bethesda, MD 20892 USA
[4] Duke Univ, Med Ctr, Div Gynecol Oncol, Durham, NC 27710 USA
关键词
D O I
10.1006/gyno.1999.5439
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objective. Our objective was to determine the frequency of allelic loss at 8p21 in sporadic epithelial ovarian cancer. We recently described allelic loss at this locus in 7/9 ovarian cancers from patients with BRCA1 gene mutations. Methods, We anonymously obtained and examined 40 unselected invasive epithelial ovarian cancers and 5 low-malignant-potential (LMP) ovarian tumors for loss of heterozygosity (LOH) at 8p12-22, Pure epithelial and stromal cell populations were procured selectively by laser capture microdissection and extracted DNA was amplified with polymorphic microsatellite markers spanning the region of interest. Results. LOH was highest (50%) at marker D8S136 located at 8p21 with 15 of 30 informative cases exhibiting an allelic deletion. None of the LMP tumors evaluated showed LOH at 8p12-22. A trend toward more frequent LOH at 8p12-22 was identified with increasing disease aggressiveness from LMP to early stage invasive ovarian cancer to advanced stage invasive ovarian cancer (Lehman's test, P-2 < 0.024). Conclusions. Fifty percent allelic loss at the distal portion of 8p21 has not been reported to date for sporadic epithelial ovarian carcinomas, The higher rate of loss in our cohort, in contrast to previous allelotyping studies, is due likely to analysis from homogenous cell populations. These results, in concert with our previous study of BRCA1 mutation-positive patients, suggest a tumor suppressor gene locus at 8p21 for epithelial ovarian cancer.
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页码:98 / 102
页数:5
相关论文
共 28 条
  • [1] Loss of heterozygosity in human ovarian cancer on chromosome 19q
    Bicher, A
    Ault, K
    Kimmelman, A
    Gershenson, D
    Reed, E
    Liang, B
    [J]. GYNECOLOGIC ONCOLOGY, 1997, 66 (01) : 36 - 40
  • [2] BOVA GS, 1993, CANCER RES, V53, P3869
  • [3] CLIBY W, 1993, CANCER RES, V53, P2393
  • [4] Cooke IE, 1996, GENE CHROMOSOME CANC, V15, P223, DOI 10.1002/(SICI)1098-2264(199604)15:4<223::AID-GCC4>3.3.CO
  • [5] 2-4
  • [6] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION
    DON, RH
    COX, PT
    WAINWRIGHT, BJ
    BAKER, K
    MATTICK, JS
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (14) : 4008 - 4008
  • [7] Identification of a 1300 kilobase deletion unit on chromosome 7q31.3 in invasive epithelial ovarian carcinomas
    Edelson, MI
    Scherer, SW
    Tsui, LC
    Welch, WR
    Bell, DA
    Berkowitz, RS
    Mok, SC
    [J]. ONCOGENE, 1997, 14 (24) : 2979 - 2984
  • [8] EMI M, 1992, CANCER RES, V52, P5368
  • [9] EMMERTBUCK MR, 1995, CANCER RES, V55, P2959
  • [10] Laser capture microdissection
    EmmertBuck, MR
    Bonner, RF
    Smith, PD
    Chuaqui, RF
    Zhuang, ZP
    Goldstein, SR
    Weiss, RA
    Liotta, LA
    [J]. SCIENCE, 1996, 274 (5289) : 998 - 1001