A Wolbachia wMel Transinfection in Aedes albopictus Is Not Detrimental to Host Fitness and Inhibits Chikungunya Virus

被引:92
作者
Blagrove, Marcus S. C. [1 ,2 ]
Arias-Goeta, Camilo [3 ]
Di Genua, Cristina [1 ,2 ]
Failloux, Anna-Bella [3 ]
Sinkins, Steven P. [1 ,2 ]
机构
[1] Univ Oxford, Oxford, England
[2] Dept Med NDM Zool, Oxford, England
[3] Inst Pasteur, Dept Virol, Paris, France
来源
PLOS NEGLECTED TROPICAL DISEASES | 2013年 / 7卷 / 03期
基金
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词
LIFE-SHORTENING WOLBACHIA; CYTOPLASMIC INCOMPATIBILITY; DENGUE TRANSMISSION; BLOCKS DENGUE; INFECTION; MOSQUITO; AEGYPTI; OUTBREAK; VECTOR; STRAIN;
D O I
10.1371/journal.pntd.0002152
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control. Methodology/Principal Findings: In Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva. Conclusions/significance: Taken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
引用
收藏
页数:7
相关论文
共 41 条
[1]  
[Anonymous], 2012, P NATL ACAD SCI US, DOI DOI 10.1073/PNAS.1204327109
[2]   Wolbachia strain wMel induces cytoplasmic incompatibility and blocks dengue transmission in Aedes albopictus [J].
Blagrove, Marcus S. C. ;
Arias-Goeta, Camilo ;
Failloux, Anna-Bella ;
Sinkins, Steven P. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (01) :255-260
[3]   INTERSPECIFIC AND INTRASPECIFIC HORIZONTAL TRANSFER OF WOLBACHIA IN DROSOPHILA [J].
BOYLE, L ;
ONEILL, SL ;
ROBERTSON, HM ;
KARR, TL .
SCIENCE, 1993, 260 (5115) :1796-1799
[4]   Chikungunya: a re-emerging virus [J].
Burt, Felicity J. ;
Rolph, Micheal S. ;
Rulli, Nestor E. ;
Mahalingam, Suresh ;
Heise, Mark T. .
LANCET, 2012, 379 (9816) :662-671
[5]   Characterization of a New Aedes albopictus (Diptera: Culicidae)-Wolbachia pipientis (Rickettsiales: Rickettsiaceae) Symbiotic Association Generated by Artificial Transfer of the wPip Strain From Culex pipiens (Diptera: Culicidae) [J].
Calvitti, Maurizio ;
Moretti, Riccardo ;
Lampazzi, Elena ;
Bellini, Romeo ;
Dobson, Stephen L. .
JOURNAL OF MEDICAL ENTOMOLOGY, 2010, 47 (02) :179-187
[6]   Natural Microbe-Mediated Refractoriness to Plasmodium Infection in Anopheles gambiae [J].
Cirimotich, Chris M. ;
Dong, Yuemei ;
Clayton, April M. ;
Sandiford, Simone L. ;
Souza-Neto, Jayme A. ;
Mulenga, Musapa ;
Dimopoulos, George .
SCIENCE, 2011, 332 (6031) :855-858
[7]   Aedes albopictus, vector of chikungunya and dengue viruses in Reunion Island:: Biology and control [J].
Delatte, H. ;
Paupy, C. ;
Dehecq, J. S. ;
Thiria, J. ;
Failloux, A. B. ;
Fontenille, D. .
PARASITE, 2008, 15 (01) :3-13
[8]   Fitness advantage and cytoplasmic incompatibility in Wolbachia single- and superinfected Aedes albopictus [J].
Dobson, SL ;
Rattanadechakul, W ;
Marsland, EJ .
HEREDITY, 2004, 93 (02) :135-142
[9]  
Dobson SL, 2002, GENETICS, V160, P1087
[10]   Chikungunya Virus and Aedes Mosquitoes: Saliva Is Infectious as soon as Two Days after Oral Infection [J].
Dubrulle, Mathieu ;
Mousson, Laurence ;
Moutailler, Sara ;
Vazeille, Marie ;
Failloux, Anna-Bella .
PLOS ONE, 2009, 4 (06)