Mutants of rat intestinal fatty acid-binding protein illustrate the critical role played by enthalpy-entropy compensation in ligand binding

被引:35
作者
Richieri, GV [1 ]
Low, PJ [1 ]
Ogata, RT [1 ]
Kleinfeld, AM [1 ]
机构
[1] MED BIOL INST,LA JOLLA,CA 92037
关键词
D O I
10.1074/jbc.272.27.16737
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Site-specific variants of rat intestinal fatty acid-binding protein were constructed to identify the molecular interactions that are important for binding to fatty acids (FAs). Several variants displayed affinities that appeared incompatible with the crystal structure of the protein-FA complex. Thermodynamic measurements provided an explanation for these apparent inconsistencies and revealed that binding affinities often inaccurately reported changes in protein-FA interactions because changes in the binding entropy and enthalpy were usually compensatory. These results demonstrate that understanding the effects of amino acid replacements on ligand binding requires measurements of enthalpy and entropy, in addition to affinity.
引用
收藏
页码:16737 / 16740
页数:4
相关论文
共 23 条
  • [1] BANASZAK L, 1994, ADV PROTEIN CHEM, V45, P89
  • [2] THE CELLULAR FATTY-ACID BINDING-PROTEINS - ASPECTS OF STRUCTURE, REGULATION, AND FUNCTION
    BASS, NM
    [J]. INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1988, 111 : 143 - 184
  • [3] Fatty acid interactions with a helix-less variant of intestinal fatty acid-binding protein
    Cistola, DP
    Kim, K
    Rogl, H
    Frieden, C
    [J]. BIOCHEMISTRY, 1996, 35 (23) : 7559 - 7565
  • [4] A HOT-SPOT OF BINDING-ENERGY IN A HORMONE-RECEPTOR INTERFACE
    CLACKSON, T
    WELLS, JA
    [J]. SCIENCE, 1995, 267 (5196) : 383 - 386
  • [5] COOK HW, 1991, BIOCH LIPIDS LIPOPRO, V20, P141
  • [6] CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS - REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL
    COWAN, SW
    NEWCOMER, ME
    JONES, TA
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1993, 230 (04) : 1225 - 1246
  • [7] Additivity principles in biochemistry
    Dill, KA
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (02) : 701 - 704
  • [8] EADS J, 1993, J BIOL CHEM, V268, P26375
  • [9] LIGAND PROTEIN ELECTROSTATIC INTERACTIONS GOVERN THE SPECIFICITY OF RETINOL-BINDING AND FATTY ACID-BINDING PROTEINS
    JAKOBY, MG
    MILLER, KR
    TONER, JJ
    BAUMAN, A
    CHENG, L
    LI, E
    CISTOLA, DP
    [J]. BIOCHEMISTRY, 1993, 32 (03) : 872 - 878
  • [10] Affinity of fatty acid for rRat intestinal fatty acid binding protein: Further examination
    Kurian, E
    Kirk, WR
    Prendergast, FG
    [J]. BIOCHEMISTRY, 1996, 35 (12) : 3865 - 3874