Probing the Aglycon Promiscuity of an Engineered Glycosyltransferase

被引：112

作者：

Gantt, Richard W. ^{[1
]}

Goff, Randal D. ^{[1
]}

Williams, Gavin J. ^{[1
]}

Thorson, Jon S. ^{[1
]}

机构：

[1] Univ Wisconsin, Sch Pharm, Lab Biosynthet Chem, UW Natl Cooperat Drug Discovery Grp, Madison, WI 53705 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 46期

基金：

美国国家卫生研究院;

关键词：

carbohydrates; enzymes; glycosides; glycosylation; natural products;

D O I：

10.1002/anie.200803508

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) A sweet library: Two variants (wild-type (WT) and a triple mutant) of glycosyltransferase (GT) OleD have been shown to catalyze glycosylation of over 70 substrates, formation of O-, S- and N-glycosidic bonds, and iterative glycosylation (see scheme). Identified substrates include nucleophiles not previously known to act in GT reactions and span numerous natural product and therapeutic drug classes. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：8889 / 8892

页数：4

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