Guard cell- and phloem idioblast-specific expression of thioglucoside glucohydrolase 1 (myrosinase) in Arabidopsis

被引:111
作者
Husebye, H
Chadchawan, S
Winge, P
Thangstad, OP
Bones, AM [1 ]
机构
[1] Norwegian Univ Sci & Technol, Dept Bot, N-7491 Trondheim, Norway
[2] Chulalongkorn Univ, Dept Bot, Bangkok 10330, Thailand
关键词
D O I
10.1104/pp.010925
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Thioglucoside glucohydrolase 1 (TGG1) is one of two known functional myrosinase enzymes in Arabidopsis. The enzyme catalyzes the hydrolysis of glucosinolates into compounds that are toxic to various microbes and herbivores. Transgenic Arabidopsis plants carrying beta-glucuronidase and green fluorescent protein reporter genes fused to 0.5 or 2.5 kb of the TGG1 promoter region were used to study spatial promoter activity. Promoter activity was found to be highly specific and restricted to guard cells and distinct cells of the phloem. No promoter activity was detected in the root or seed. All guard cells show promoter activity. Positive phloem cells are distributed in a discontinuous pattern and occur more frequent in young tissues. Immunocytochemical localization of myrosinase in transverse and longitudinal sections of embedded material show that the TGG1 promoter activity reflects the position of the myrosinase enzyme. In the flower stalk, the myrosinase-containing phloem cells are located between phloem sieve elements and glucosinolate-rich S cells. Our results suggest a cellular separation of myrosinase enzyme and glucosinolate substrate, and that myrosinase is contained in distinct cells. We discuss the potential advantages of locating defense and communication systems to only a few specific cell types.
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页码:1180 / 1188
页数:9
相关论文
共 36 条
[1]  
BONES A, 1985, ISRAEL J BOT, V34, P351
[2]  
Bones AM, 1996, PHYSIOL PLANTARUM, V97, P194, DOI 10.1111/j.1399-3054.1996.tb00497.x
[3]   FATE OF MYROSIN CELLS - CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST MYROSINASE [J].
BONES, AM ;
THANGSTAD, OP ;
HAUGEN, OA ;
ESPEVIK, T .
JOURNAL OF EXPERIMENTAL BOTANY, 1991, 42 (245) :1541-1549
[4]   Spatial organization of the glucosinolate-myrosinase system in brassica specialist aphids is similar to that of the host plant [J].
Bridges, M ;
Jones, AME ;
Bones, AM ;
Hodgson, C ;
Cole, R ;
Bartlet, E ;
Wallsgrove, R ;
Karapapa, VK ;
Watts, N ;
Rossiter, JT .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2002, 269 (1487) :187-191
[5]   ARABIDOPSIS CDNA SEQUENCE ENCODING MYROSINASE [J].
CHADCHAWAN, S ;
BISHOP, J ;
THANGSTAD, OP ;
BONES, AM ;
MITCHELLOLDS, T ;
BRADLEY, D .
PLANT PHYSIOLOGY, 1993, 103 (02) :671-672
[6]  
CHEW FS, 1998, CHEM MEDIATION COEVO, P45
[7]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[8]  
Cole RA, 1997, ENTOMOL EXP APPL, V85, P121, DOI 10.1023/A:1003049105464
[9]   CHARACTERIZATION OF A NEW MYROSINASE IN BRASSICA-NAPUS [J].
FALK, A ;
EK, B ;
RASK, L .
PLANT MOLECULAR BIOLOGY, 1995, 27 (05) :863-874
[10]   Purification and characterisation of epithiospecifier protein from Brassica napus:: enzymic intramolecular sulphur addition within alkenyl thiohydroximates derived from alkenyl glucosinolate hydrolysis [J].
Foo, HL ;
Gronning, LM ;
Goodenough, L ;
Bones, AM ;
Danielsen, BE ;
Whiting, DA ;
Rossiter, JT .
FEBS LETTERS, 2000, 468 (2-3) :243-246