Structure and cross-reactivity of the O-antigen of Proteus vulgaris O8

被引：12

作者：

Perepelov, AV

Babicka, D

Shashkov, AS

Arbatsky, NP

Senchenkova, SN

Rozalski, A

Knirel, YA

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 117913, Russia

[2] Univ Lodz, Inst Microbiol & Immunol, PL-90237 Lodz, Poland

来源：

CARBOHYDRATE RESEARCH | 1999年 / 318卷 / 1-4期

基金：

俄罗斯基础研究基金会;

关键词：

Proteus vulgaris; lipopolysaccharide; O-specific polysaccharide structure; serological cross-reactivity;

D O I：

10.1016/S0008-6215(99)00101-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-molecular-mass O-specific polysaccharide was obtained by mild acid degradation of Proteus vulgaris O8 lipopolysaccharide followed by gel permeation chromatography. Studies of the polysaccharide by sugar and methylation analyses and H-1 and C-13 NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and PI-detected H-1, C-13 heteronuclear multiple-quantum coherence (HMQC) experiments, demonstrated the presence of a tetrasaccharide repeating unit having the following structure: alpha-D-Galp 1 down arrow 3 --> 3)-beta-D-GlcpA-(1 --> 4)-alpha-L-FucpNAc-(1 --> 3)-alpha-D-GlcpNAc-(1 --> The role of an epitope associated with the alpha-L-FucpNAc-(1 --> 3)-D-GlcpNAc disaccharide in serological cross-reactivity of P. vulgaris O8 is discussed. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：186 / 192

页数：7

共 23 条

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[Anonymous], 1972, METHODS CARBOHYDR CH

[2] Structural and serological studies on a new acidic O-specific polysaccharide of Proteus vulgaris O32 [J].