Dielectrophoretic separation of bacteria using a conductivity gradient

被引:169
作者
Markx, GH
Dyda, PA
Pethig, R
机构
[1] Inst. of Molec./Biomol. Electronics, University of Wales, Bangor, Gwynedd LL57 1UT, Dean Street
关键词
bacteria; cell separation; conductivity; dielectrophoresis;
D O I
10.1016/0168-1656(96)01617-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Dielectrophoresis, the lateral motion induced on particles by non-uniform electric fields, is a sensitive function of the electrical conductivity of the particle suspending medium. This dependence is exploited in a new technique for separating bioparticles from suspended mixtures. The bioparticles are first immobilised by positive dielectrophoresis at electrodes in a separation chamber, and the conductivity of the liquid flowing through the chamber is then gradually and continuously increased so as to produce a conductivity gradient with time. The bioparticles are released from the electrodes according to their own dielectric properties and as a function of flow rate and medium conductivity. This is demonstrated for pure suspensions and mixtures of the bacteria Bacillus subtilis, Escherichia coli and Micrococcus luteus.
引用
收藏
页码:175 / 180
页数:6
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