Typing of bovine viral diarrhea viruses directly from blood of persistently infected cattle by multiplex PCR

被引：56

作者：

Gilbert, SA ^{[1
]}

Burton, KM ^{[1
]}

Prins, SE ^{[1
]}

Deregt, D ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Anim Dis Res Inst, Lethbridge, AB T1J 3Z4, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 06期

关键词：

D O I：

10.1128/JCM.37.6.2020-2023.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A nested multiplex PCR was developed for genotyping of bovine viral diarrhea viruses (BVDVs). The assay could detect as little as 3 50% tissue culture infective doses of BVDV per mi and typed 42 out of 42 cell culture isolates. BVDV was also successfully typed, with or without RNA extraction, from all 27 whole-blood samples examined from 22 carriers or probable carriers and 5 experimentally infected cattle.

引用

页码：2020 / 2023

页数：4

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