Stable delivery of a canavalin promoter-beta-glucuronidase gene fusion into French bean by particle bombardment

被引：6

作者：

Kim, JW

Minamikawa, T

机构：

[1] Department of Biology, Tokyo Metropolitan University, Hachioji, Tokyo

来源：

PLANT AND CELL PHYSIOLOGY | 1997年 / 38卷 / 01期

关键词：

canavalin::GUS gene; GUS activity; particle bombardment; Phaseolus vulgaris; transgenic plants;

D O I：

10.1093/oxfordjournals.pcp.a029087

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Shoot apices of French bean (Phaseolus vulgaris L. cv. Goldstar) seeds were bombarded with gold particles coated with plasmid pSAG-734 carrying a chimeric gene that encodes beta-glucuronidase under the control of the canavalin gene promoter. The organ- and maturation stage-specific expression of the construct was observed histochemically in seeds of transgenic plants.

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收藏

页码：70 / 75

页数：6

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