Global synthetic-lethality analysis and yeast functional profiling

被引：115

作者：

Ooi, SL ^{[1
]}

Pan, XW ^{[1
]}

Peyser, BD ^{[1
]}

Ye, P ^{[1
]}

Meluh, PB ^{[1
]}

Yuan, DS ^{[1
]}

Irizarry, RA ^{[1
]}

Bader, JS ^{[1
]}

Spencer, FA ^{[1
]}

Boeke, JD ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, High Throughput Biol Ctr, Inst Med Genet,Dept Biostat, Baltimore, MD 21205 USA

来源：

TRENDS IN GENETICS | 2006年 / 22卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.tig.2005.11.003

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

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收藏

页码：56 / 63

页数：8

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