Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit

被引：8

作者：

Choudhurry, Roos S. R. ^{[1
]}

Melles, Damian C. ^{[1
]}

Eadie, Kimberly ^{[1
]}

Vos, Margreet ^{[1
]}

Wertheim, Heiman F. L. ^{[1
]}

Verbrugh, Henry A. ^{[1
]}

van Belkum, Alex ^{[1
]}

van Leeuwen, Willem B. ^{[1
]}

机构：

[1] Erasmus MC, Dept Med Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 65卷 / 02期

关键词：

direct detection; S. aureus colonisation; real-time PCR;

D O I：

10.1016/j.mimet.2005.08.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Lightcycler Staphylococcus kit((R)) is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：354 / 356

页数：3

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