Determination of atropine in pharmaceutical preparations by liquid chromatography with fluorescence detection

被引：28

作者：

Takahashi, M

Nagashima, M

Shigeoka, S

Nishijima, M

Kamata, K

机构：

[1] Tokyo Metropol. Res. Lab. Pub. Hlth., 24-1, Hyakunincho, 3-chome, S.

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 775卷 / 1-2期

关键词：

pharmaceutical analysis; derivatization; LC; atropine; alkaloids; 1-anthroylnitrile;

D O I：

10.1016/S0021-9673(97)00305-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the determination of atropine in pharmaceutical preparations by precolumn derivatization and high-performance liquid chromatography (HPLC). Atropine is derivatized by reacting with 1-anthroylnitrile (1-AN) in acetone. The 1-AN derivative of atropine is then used for quantitative HPLC analysis on a reversed-phase column with fluorescence detection. The calibration graph was linear over a sample concentration range from 0.05 to 1.0 mu g/ml; the limit of detection was reached at a sample concentration of 0.01 mu g/ml. The proposed method was satisfactorily applied to the determination of atropine in pharmaceutical preparations. (C) 1997 Elsevier Science B.V.

引用

页码：137 / 141

页数：5

共 20 条

[1] SPECTROPHOTOMETRIC DETERMINATION OF ATROPINE
AMAN, T
HASSAN, A
KHOKHAR, I
RASHID, A
[J]. ANALYTICAL LETTERS, 1994, 27 (10) : 1833 - 1845
[2] [Anonymous], 1995, US PHARM, P145
[3] REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DATABASE OF RETENTION INDEXES AND UV SPECTRA OF TOXICOLOGICALLY RELEVANT SUBSTANCES AND ITS INTERLABORATORY USE
BOGUSZ, M
ERKENS, M
[J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 674 (1-2) : 97 - 126
[4] Buech U., 1994, Methods and Findings in Experimental and Clinical Pharmacology, V16, P361
[5] CIERI UR, 1978, J ASSOC OFF ANA CHEM, V61, P937
[6] EVIDENCE OF TRANSACYLATION OF SCOPOLAMINE IN THE GAS-CHROMATOGRAPH INLET
DEUTSCH, J
HEGEDUS, L
GREIG, NH
RAPOPORT, SI
SONCRANT, TT
[J]. ANALYTICAL LETTERS, 1994, 27 (04) : 671 - 679
[7] THIN-LAYER CHROMATOGRAPHY UNDER TROPICAL CONDITIONS - IMPACT OF HIGH-TEMPERATURES AND HIGH HUMIDITIES ON SCREENING SYSTEMS FOR ANALYTICAL TOXICOLOGY
DEZEEUW, RA
FRANKE, JP
VANHALEM, M
SCHAAPMAN, S
LOGAWA, E
SIREGAR, CJP
[J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 664 (02) : 263 - 270
[8] ELSHABOURI SR, 1985, B PHARM SCI, V8, P120
[9] GOLKIEWICZ W, 1994, J CHROMATOGR A, V686, P85
[10] STUDIES ON STEROIDS .187. DETERMINATION OF SERUM BILE-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE LABELING
GOTO, J
SAITO, M
CHIKAI, T
GOTO, N
NAMBARA, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1983, 276 (02): : 289 - 300

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