Assignment of Rfp-Y to the chicken major histocompatibility complex NOR microchromosome and evidence for high-frequency recombination associated with the nucleolar organizer region

被引:136
作者
Miller, MM
Goto, RM
Taylor, RL
Zoorob, R
Auffray, C
Briles, RW
Briles, WE
Bloom, SE
机构
[1] UNIV NEW HAMPSHIRE,DEPT ANIM & NUTR SCI,DURHAM,NH 03824
[2] UPR 420,CNRS,F-94801 VILLEJUIF,FRANCE
[3] NO ILLINOIS UNIV,DEPT BIOL SCI,DE KALB,IL 60115
[4] CORNELL UNIV,DEPT MICROBIOL & IMMUNOL,ITHACA,NY 14853
关键词
trisomy mapping; ribosomal RNA genes;
D O I
10.1073/pnas.93.9.3958
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Rfp-Y is a second region in the genome of the chicken containing major histocompatibility complex (MHC) class I and II genes. Haplotypes of Rfp-Y assort independently from haplotypes of the B system, a region known to function as a MHC and to be located on chromosome 16 (a microchromosome) with the single nucleolar organizer region (NOR) in the chicken genome. Linkage mapping with reference populations failed to reveal the location of Rfp-Y, leaving Rfp-Y unlinked in a map containing >400 markers. A possible location of Rfp-Y became apparent in studies of chickens trisomic for chromosome 16 when it was noted that the intensity of restriction fragments associated with Rfp-Y increased with increasing copy number of chromosome 16. Further evidence that Rfp-Y might be located on chromosome 16 was obtained when individuals trisomic for chromosome 16 were found to transmit three Rfp-Y haplotypes. Finally, mapping of cosmid cluster LII of the molecular map of chicken R-IHC genes (containing a MHC class II gene and two rRNA genes) to Rfp-Y validated the assignment of Rfp-Y to the MHC/NOR microchromosome. A genetic map can now be drawn for a portion of chicken chromosome 16 with Rfp-Y, encompassing two MHC class I and three MHC class II genes, separated from the B system by a region containing the NOR and exhibiting highly frequent recombination.
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页码:3958 / 3962
页数:5
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