Molecular cloning of a peroxidase mRNA specifically expressed in the ink gland of Sepia officinalis

被引：19

作者：

Gesualdo, I ^{[1
]}

Aniello, F ^{[1
]}

Branno, M ^{[1
]}

Palumbo, A ^{[1
]}

机构：

[1] Staz Zool Anton Dohrn, LAB BIOCHEM & MOL BIOL, I-80121 NAPLES, ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1353卷 / 02期

关键词：

peroxidase; ink gland; melanogenesis; (Sepia officinalis);

D O I：

10.1016/S0167-4781(97)00088-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA encoding a peroxidase has been assembled from two different overlapping cDNA clones isolated from the cDNA library of Sepia officinalis ink gland and 5' RACE procedure. The cDNA of 2898 bp contains an open reading frame of 2778 bp corresponding to a protein of 926 amino acids. The protein shows 76% identity with a peroxidase isolated from the light organ of the squid Euprymna scolopes and approximately 30% identity with mammalian peroxidases. Northern blot analysis of mRNA from various tissues of Sepia officinalis reveals that peroxidase is exclusively expressed in the ink gland. These results represent the first characterization of a melanogenic peroxidase whose possible involvement in the biosynthesis of melanin is discussed. (C) 1997 Elsevier Science B.V.

引用

页码：111 / 117

页数：7

共 28 条

[1]

CHAKRABORTY AK, 1996, EUR J BIOCHEM, V232, P257

[2]

CHOMCZYNSKI P, 1897, ANN BIOCH, V162, P156

[3] PEROXIDASE AS AN ALTERNATIVE TO TYROSINASE IN THE OXIDATIVE POLYMERIZATION OF 5,6-DIHYDROXYINDOLES TO MELANIN(S) [J].

DISCHIA, M ;

NAPOLITANO, A ;

PROTA, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1073 (02) :423-430

[4] ENZYMATIC CONTROL OF PIGMENTATION IN MAMMALS [J].

HEARING, VJ ;

TSUKAMOTO, K .

FASEB JOURNAL, 1991, 5 (14) :2902-2909

[5]

HERRING PJ, 1988, MOLLUSCA, V1, P449

[6]

JIMENEZCERVANTE.C, 1994, J BIOL CHEM, V269, P17992

[7] CHARACTERIZATION OF CDNA CLONES FOR HUMAN MYELOPEROXIDASE - PREDICTED AMINO-ACID-SEQUENCE AND EVIDENCE FOR MULTIPLE MESSENGER-RNA SPECIES [J].

JOHNSON, KR ;

NAUSEEF, WM ;

CARE, A ;

WHEELOCK, MJ ;

SHANE, S ;

HUDSON, S ;

KOEFFLER, HP ;

SELSTED, M ;

MILLER, C ;

ROVERA, G .

NUCLEIC ACIDS RESEARCH, 1987, 15 (05) :2013-2028

[8] HUMAN THYROID PEROXIDASE - COMPLETE CDNA AND PROTEIN-SEQUENCE, CHROMOSOME MAPPING, AND IDENTIFICATION OF 2 ALTERNATELY SPLICED MESSENGER-RNAS [J].

KIMURA, S ;

KOTANI, T ;

MCBRIDE, OW ;

UMEKI, K ;

HIRAI, K ;

NAKAYAMA, T ;

OHTAKI, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5555-5559

[9]

Klebanoff SJ, 1991, PEROXIDASES CHEM BIO, V1, P1

[10]

MORISHITA K, 1987, J BIOL CHEM, V262, P15208

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