Effect of vector-expressed shRNAs on hTERT expression

被引：12

作者：

Guo, Ying ^{[2
]}

Liu, Jun ^{[1
,2
]}

Li, Ying-Hui ^{[1
]}

Song, Tian-Bao ^{[3
]}

Wu, Jing

Zheng, Cai-Xia ^{[2
]}

Xue, Cai-Fang ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Dept Etiol, Xian 710033, Shaanxi Provinc, Peoples R China

[2] Xi An Jiao Tong Univ, Hosp 2, Dept Obstet & Gynecol, Xian 710001, Shaanxi Provinc, Peoples R China

[3] Xi An Jiao Tong Univ, Sch Med, Dept Histol & Embryol, Xian 710061, Shaanxi Provinc, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2005年 / 11卷 / 19期

关键词：

RNA interference; Short hairpin RNA; Telomerase;

D O I：

10.3748/wjg.v11.i19.2912

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To study the effect of short hairpin RNAs (shRNAs) expressed from DNA vector on hTERT expression. METHODS: Oligonucleotides coding for four shRNAs against hTERT were cloned into a mammalian shRNA expression vector pUC18U6 to form pUC18U6ht1-4, which were then introduced into HepG2 cells by using liposome-mediated transfection. HepG2 cells transfected by pUC18U6 and pUC18U6GFPsir, which expressed shRNA against green fluorescent protein (GFP), were used as controls. hTERT mRNA in the transfected cells were quantified by using real-time fluorescent RT-PCR. RESULTS: Among the four shRNAs against hTERT, two decreased the hTERT mRNA level. Compared with the controls, pUC18U6ht which expressed the two shRNAs reduced hTERT mRNA by 39% and 49% (P<0.05). CONCLUSION: hTERT expression is inhibited by the shRNAs expressed from the DNA vector. (c) 2005 The WJG Press and Elsevier Inc. All rights reserved.

引用

页码：2912 / 2915

页数：4

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