Assessment of real-time PCR based methods for quantification of pollen-mediated gene flow from GM to conventional maize in a field study

被引:60
作者
Pla, M
La Paz, JL
Peñas, G
García, N
Palaudelmàs, M
Esteve, T
Messeguer, J
Melé, E
机构
[1] Univ Girona, INTEA, Escola Politecn Super, Girona 17071, Spain
[2] Consorci CSIC, IRTA, IBMB, ES-08034 Barcelona, Spain
[3] Consorci CSIC, IRTA, Dept Genet Vegetal, ES-08034 Barcelona, Spain
关键词
coexistence; cross-pollination; field study; genetically modified organism GMO; maize; Mon810; real-time PCR;
D O I
10.1007/s11248-005-4945-x
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Maize is one of the main crops worldwide and an increasing number of genetically modified (GM) maize varieties are cultivated and commercialized in many countries in parallel to conventional crops. Given the labeling rules established e.g. in the European Union and the necessary coexistence between GM and non-GM crops, it is important to determine the extent of pollen dissemination from transgenic maize to other cultivars under field conditions. The most widely used methods for quantitative detection of GMO are based on real-time PCR, which implies the results are expressed in genome percentages (in contrast to seed or grain percentages). Our objective was to assess the accuracy of real-time PCR based assays to accurately quantify the contents of transgenic grains in non-GM fields in comparison with the real cross-fertilization rate as determined by phenotypical analysis. We performed this study in a region where both GM and conventional maize are normally cultivated and used the predominant transgenic maize Mon810 in combination with a conventional maize variety which displays the characteristic of white grains (therefore allowing cross-pollination quantification as percentage of yellow grains). Our results indicated an excellent correlation between real-time PCR results and number of cross-fertilized grains at Mon810 levels of 0.1-10%. In contrast, Mon810 percentage estimated by weight of grains produced less accurate results. Finally, we present and discuss the pattern of pollen-mediated gene flow from GM to conventional maize in an example case under field conditions.
引用
收藏
页码:219 / 228
页数:10
相关论文
共 30 条
[1]  
Arthur KM, 2003, GENETICS, V165, P2137
[2]  
BENETRIX F, 2003, PERSPECTIVES AGRICOL
[3]  
BROOKES G, 2004, PC EC
[4]  
Emberlin J., 1999, REPORT DISPERSAL MAI
[5]  
HENRY C, 2003, 15138 EPG DEFRA
[6]   Development of real-time PCR systems based on SYBR® Green I, Amplifluor™ and TaqMan® technologies for specific quantitative detection of the transgenic maize event GA21 [J].
Hernández, M ;
Esteve, T ;
Prat, S ;
Pla, M .
JOURNAL OF CEREAL SCIENCE, 2004, 39 (01) :99-107
[7]   A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard® based on the 3′-transgene integration sequence [J].
Hernández, M ;
Pla, M ;
Esteve, T ;
Prat, S ;
Puigdomènech, P ;
Ferrando, A .
TRANSGENIC RESEARCH, 2003, 12 (02) :179-189
[8]   Development and comparison of four real-time polymerase chain reaction systems for specific detection and quantification of Zea mays L. [J].
Hernández, M ;
Duplan, MN ;
Berthier, G ;
Vaïtilingom, M ;
Hauser, W ;
Freyer, R ;
Pla, M ;
Bertheau, Y .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (15) :4632-4637
[9]   5′-nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize [J].
Holck, A ;
Va, M ;
Didierjean, L ;
Rudi, K .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2002, 214 (05) :449-453
[10]   DETECTION OF SPECIFIC POLYMERASE CHAIN-REACTION PRODUCT BY UTILIZING THE 5'-]3' EXONUCLEASE ACTIVITY OF THERMUS-AQUATICUS DNA-POLYMERASE [J].
HOLLAND, PM ;
ABRAMSON, RD ;
WATSON, R ;
GELFAND, DH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7276-7280