Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics

被引:359
作者
Foudeh, Amir M. [1 ]
Didar, Tohid Fatanat [1 ]
Veres, Teodor [1 ,2 ]
Tabrizian, Maryam [1 ]
机构
[1] McGill Univ, Dept Biomed Engn, Montreal, PQ H3A 2B4, Canada
[2] Natl Res Council Canada, Boucherville, PQ J4B 6Y4, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
POLYMERASE-CHAIN-REACTION; ESCHERICHIA-COLI O157H7; MOLECULARLY IMPRINTED POLYMERS; ROLLING-CIRCLE AMPLIFICATION; REAL-TIME PCR; RAPID DETECTION; ELECTRICAL DETECTION; DNA EXTRACTION; SAMPLE PREPARATION; GENETIC-ANALYSIS;
D O I
10.1039/c2lc40630f
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Effective pathogen detection is an essential prerequisite for the prevention and treatment of infectious diseases. Despite recent advances in biosensors, infectious diseases remain a major cause of illnesses and mortality throughout the world. For instance in developing countries, infectious diseases account for over half of the mortality rate. Pathogen detection platforms provide a fundamental tool in different fields including clinical diagnostics, pathology, drug discovery, clinical research, disease outbreaks, and food safety. Microfluidic lab-on-a-chip (LOC) devices offer many advantages for pathogen detection such as miniaturization, small sample volume, portability, rapid detection time and point-of-care diagnosis. This review paper outlines recent microfluidic based devices and LOC design strategies for pathogen detection with the main focus on the integration of different techniques that led to the development of sample-to-result devices. Several examples of recently developed devices are presented along with respective advantages and limitations of each design. Progresses made in biomarkers, sample preparation, amplification and fluid handling techniques using microfluidic platforms are also covered and strategies for multiplexing and high-throughput analysis, as well as point-of-care diagnosis, are discussed.
引用
收藏
页码:3249 / 3266
页数:18
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