Influenza B Virus NS1-Truncated Mutants: Live-Attenuated Vaccine Approach

被引：91

作者：

Hai, Rong ^{[1
]}

Martinez-Sobrido, Luis ^{[1
,3
]}

Fraser, Kathryn A. ^{[1
]}

Ayllon, Juan ^{[4
]}

Garcia-Sastre, Adolfo ^{[1
,2
,3
]}

Palese, Peter ^{[1
,2
]}

机构：

[1] Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA

[2] Mt Sinai Sch Med, Dept Med, New York, NY 10029 USA

[3] Mt Sinai Sch Med, Emerging Pathogens Inst, New York, NY 10029 USA

[4] Univ Salamanca, Dept Bioquim & Biol Mol, E-37008 Salamanca, Spain

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 21期

关键词：

D O I：

10.1128/JVI.01213-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Type B influenza viruses can cause substantial morbidity and mortality in the population, and vaccination remains by far the best means of protection against infections with these viruses. Here, we report the construction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates. Employing reverse genetics, we altered the NS1 gene, which encodes a type I interferon (IFN) antagonist. The resulting NS1 mutant viruses induced IFN and, as a consequence, were found to be attenuated in vitro and in vivo. The absence of pathogenicity of the NS1 mutants in both BALB/c and C57BL/6 PKR-/- mice was confirmed. We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immune response and confer effective protection against challenge with both homologous and heterologous B virus strains in mice.

引用

页码：10580 / 10590

页数：11

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