Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein - Nitrilotriacetic acid conjugate

被引：81

作者：

Goldsmith, CR

Jaworski, J

Sheng, M

Lippard, SJ ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] MIT, Picower Ctr Learning & Memory, Howard Hughes Med Inst, Cambridge, MA 02139 USA

[3] MIT, RIKEN Mit Neurosci Res Ctr, Cambridge, MA 02139 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 02期

关键词：

D O I：

10.1021/ja0559754

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The compound NTA-DCF consists of two components, a dichlorofluorescein (DCF) reporter and a nitrilotriacetic acid (NTA) functionality. The latter binds polyhistidine sequences selectively through a bridging metal ion. The NTA-DCF conjugate has photophysical properties similar to those of the parent DCF fluorophore both by itself and as its nickel(II) complex. The insensitivity of the emission to paramagnetic ions allows the probe to label His6-tagged proteins fluorescently on the extracellular surfaces of HEK 293-T and HeLa cells. Copyright © 2006 American Chemical Society.

引用

页码：418 / 419

页数：2

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