Plant cell growth and differentiation may involve GAP regulation of Rac activity

被引：29

作者：

Borg, S ^{[1
]}

Podenphant, L ^{[1
]}

Jensen, TJ ^{[1
]}

Poulsen, C ^{[1
]}

机构：

[1] Aarhus Univ, Dept Biol Mol & Struct, Gene Express Lab, DK-8000 Aarhus C, Denmark

来源：

FEBS LETTERS | 1999年 / 453卷 / 03期

关键词：

two-hybrid screening; fusion protein expression; GTPase activating protein; Cdc42/Rac interactive binding element; root nodule development; Lotus japonicus;

D O I：

10.1016/S0014-5793(99)00750-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two Pac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus, Two-hybrid screening with dominant-constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho-GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules, (C) 1999 Federation of European Biochemical Societies.

引用

页码：341 / 345

页数：5

共 25 条

[1] Ausubel FM, 1995, SHORT PROTOCOLS MOL
[2] A NOVEL STRATEGY FOR PRODUCTION OF A HIGHLY EXPRESSED RECOMBINANT PROTEIN IN AN ACTIVE FORM
BLACKWELL, JR
HORGAN, R
[J]. FEBS LETTERS, 1991, 295 (1-3) : 10 - 12
[3] PROTEINS REGULATING RAS AND ITS RELATIVES
BOGUSKI, MS
MCCORMICK, F
[J]. NATURE, 1993, 366 (6456) : 643 - 654
[4] Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotos japonicus, and expression of corresponding mRNAs in developing root nodules
Borg, S
Brandstrup, B
Jensen, TJ
Poulsen, C
[J]. PLANT JOURNAL, 1997, 11 (02) : 237 - 250
[5] BORGS P, 1994, LYMPHOLOGY, V27, P175
[6] Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast
Brown, JL
Jaquenoud, M
Gulli, MP
Chant, J
Peter, M
[J]. GENES & DEVELOPMENT, 1997, 11 (22) : 2972 - 2982
[7] GTPASE CASCADES CHOREOGRAPHING CELLULAR BEHAVIOR - MOVEMENT, MORPHOGENESIS, AND MORE
CHANT, J
STOWERS, L
[J]. CELL, 1995, 81 (01) : 1 - 4
[8] ROLES OF PLANT HOMOLOGS OF RAB1P AND RAB7P IN THE BIOGENESIS OF THE PERIBACTEROID MEMBRANE, A SUBCELLULAR COMPARTMENT FORMED DE-NOVO DURING ROOT-NODULE SYMBIOSIS
CHEON, CI
LEE, NG
SIDDIQUE, ABM
BAL, AK
VERMA, DPS
[J]. EMBO JOURNAL, 1993, 12 (11) : 4125 - 4135
[9] Deutscher M, 1990, METHODS ENZYMOLOGY, V182
[10] RAC GTPASE INTERACTS WITH GAPS AND TARGET PROTEINS THROUGH MULTIPLE EFFECTOR SITES
DIEKMANN, D
NOBES, CD
BURBELO, PD
ABO, A
HALL, A
[J]. EMBO JOURNAL, 1995, 14 (21) : 5297 - 5305

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