Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

被引：23

作者：

Deng, JZ

Starck, SR

Hecht, SM

Ijames, CF

Hemling, ME

机构：

[1] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA

[2] Univ Virginia, Dept Biol, Charlottesville, VA 22901 USA

[3] SmithKline Beecham Pharmaceut, Dept Phys & Struct Chem, King Of Prussia, PA 19406 USA

来源：

JOURNAL OF NATURAL PRODUCTS | 1999年 / 62卷 / 07期

关键词：

D O I：

10.1021/np990099r

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bioassay-guided fractionation of an active methyl ethyl ketone extract of Hardwickia binata, using an assay sensitive to DNA polymerase beta inhibition, resulted in the isolation of a potent inhibitor. This proved to be a novel diterpenoid, which has been named harbinatic acid (1). The structure of 1 was established as 3 alpha-O-trans-p-coumaroyl-7-labden-15-oic acid from spectroscopic analysis and by comparison with the published data for a structurally related compound. Compound 1 strongly inhibited calf thymus PNA polymerase beta, with an IC50 value of 2.9 mu M.

引用

页码：1000 / 1002

页数：3

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