Evidence of absence of porcine endogenous retrovirus (PERV) infection in patients treated with a bioartificial liver support system

被引：116

作者：

Pitkin, Z ^{[1
]}

Mullon, C ^{[1
]}

机构：

[1] Circe Biomed Inc, Lexington, MA 02421 USA

来源：

ARTIFICIAL ORGANS | 1999年 / 23卷 / 09期

关键词：

bioartificial liver; PERV; porcine hepatocytes; xenotransplantation;

D O I：

10.1046/j.1525-1594.1999.06444.x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Porcine endogenous retrovirus (PERV) genomes are present in all pig cells. In this retrospective study, we assessed PERV infectivity in 28 patients treated with an extracorporeal bioartificial liver (HepatAssist system) that includes a membrane device containing porcine hepatocytes. All patients tested negative for PERV using polymerase chain reaction analysis of peripheral blood mononuclear cells (PBMC) collected up to 5 years after treatment. In vitro results showed that the membrane decreased the risk of PERV transmission by a factor of 10(5), and porcine hepatocytes did not produce infectious PERV in co-cultures with human cell line 293. Our results do not support the presence of PERV infection in patients treated with this porcine hepatocyte-based bioartificial liver.

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页码：829 / 833

页数：5

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