Neutrophil recruitment by interleukin-17 into rat airways in vivo -: Role of tachykinins

We determined whether neutrophil recruitment induced by the T-lymphocyte cytokine, interleukin-17 (IL-17) is modulated by tachykinins in airways in vivo. Cell recruitment into airways was induced by either human (h) IL-17 (1 mu g) or rat (r) IL-1 beta (2.5 ng), instilled intratracheally in rats (n = 5 to 7), Six hours after instillation, hlL-17 (3.1 +/- 1.2 x 10(6) cells/ml) and rIL-1 beta (4.1 +/- 0.5 x 10(6) cells/ml), respectively, induced a significant and selective increase in neutrophil count in bronchoalveolar lavage fluid (BAL) when compared with vehicle (0.6 +/- 0.2 x 10(6) cells/ml). For hlL-17, this effect was dose-dependent. Inhalation of peptidase inhibitors (phosphoramidon plus captopril) potentiated the effect of both hIL-17 and rIL-1 beta, Inhalation of a neutral endopeptidase inhibitor (phosphoramidon) alone also increased the neutrophil count for hIL-17, whereas an angiotensin-converting enzyme inhibitor (captopril) alone did not. A selective neurokinin (NK)-1 receptor antagonist (SR 140333) reduced the neutrophil count, both with and without phosphoramidon pretreatment. In conclusion, IL-17 selectively recruits neutrophils into rat airways in vivo and this effect is modulated by endogenous tachykinins acting via NK-1 receptors.