Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma

被引：40

作者：

Altanerova, U. ^{[1
]}

Benejova, K. ^{[1
]}

Altanerova, V. ^{[1
,2
]}

Tyciakova, S. ^{[2
]}

Rychly, B. ^{[3
]}

Szomolanyi, P. ^{[4
]}

Ciampor, F. ^{[5
]}

Cihova, M. ^{[2
]}

Repiska, V. ^{[6
]}

Ondicova, K. ^{[7
]}

Mravec, B. ^{[7
]}

Altaner, C. ^{[1
,2
]}

机构：

[1] Ctr Cell Therapy & Regenerat Med, St Elisabeth Canc Inst, Bratislava, Slovakia

[2] Slovak Acad Sci, Biomed Ctr, Canc Res Inst, Bratislava, Slovakia

[3] Cytopathos Ltd, Bratislava, Slovakia

[4] Med Univ Vienna, Dept Biomed Imaging & Image Guided Therapy, Vienna, Austria

[5] Slovak Acad Sci, Biomed Ctr, Inst Virol, Bratislava, Slovakia

[6] Comenius Univ, Fac Med, Inst Med Biol Genet & Clin Genet, Bratislava, Slovakia

[7] Comenius Univ, Fac Med, Inst Pathophysiol, Bratislava, Slovakia

来源：

NEOPLASMA | 2016年 / 63卷 / 06期

关键词：

dental pulp MSCs; iron labeling; yCD::UPRT-exosomes; intranasal application; migration to intracerebral glioblastoma; CANCER GENE-THERAPY; STEM-CELLS; ADIPOSE-TISSUE; BONE-MARROW; CAPABILITY; DELIVERY;

D O I：

10.4149/neo_2016_611

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is a drug approved for intravenous application to treat iron deficiency anemia in patients. Venofer labeling did not affect DP-MSCs or yCD::UPRT-DP-MSCs viability and growth kinetics. Electron microscopy of labeled cells showed internalized Venofer nano particles in endosomes. MRI relativity measurement of Venofer labeled DP-MSCs in a phantom arrangement revealed that 100 cells per 0.1 ml were still detectable. DP-MSCs or yCD::UPRT-DP-MSCs and the corresponding Venofer labeled cells release exosomes into conditional medium (CM). CM from yCD::UPRT-DP-MSCs in the presence of a prodrug 5-fluorocytosine caused tumor cell death in a dose dependent manner. Iron labeled DP-MSCs or yCD::UPRT-DP-MSCs sustained their tumor tropism in vivo; intra-nasally applied cells migrated and specifically engrafted orthotopic glioblastoma xenografts in rats.

引用

页码：925 / 933

页数：9

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