HSP90 function is required for morphogenesis in ascidian and echinoid embryos

被引:18
作者
Bishop, CD
Bates, WR
Brandhorst, BP [1 ]
机构
[1] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada
[2] Okanagan Univ Coll, Dept Biol, Kelowna, BC V1V 1V7, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
molecular chaperone; gastrulation; mesenchyme; epithelium;
D O I
10.1007/s00427-002-0212-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Treatment of embryos of the ascidians Boltenia villosa and Cnemidocarpa finmarkiensis and the sea urchin Strongylocentrotus purpuratus with the anti-HSP90 drugs geldanamycin and radicicol caused morphogenetic arrest. All embryonic stages during which obvious morphogenesis was observed were sensitive to treatment, including formation of the sea urchin blastular epithelium. Arrested embryos were viable for many hours to days post-treatment, indicating a low general toxicity of these drugs. Morphogenetic movements including gastrulation and migration (but not ingression) of sea urchin primary and secondary mesenchyme cells were arrested 8-10 h after treatment began. Cell division and developmentally regulated expression of some genes continued after morphogenesis was arrested. Anti-HSP90 drugs cause selective inactivation or degradation of proteins with which the protein chaperone HSP90 interacts. Therefore, morphogenetic arrest subsequent to the disruption of HSP90 function may result from the reduction in concentration, or activity, of client proteins required for morphogenetic movements of cells. The use of these drugs may provide a means to identify novel activities or proteins involved in morphogenesis.
引用
收藏
页码:70 / 80
页数:11
相关论文
共 52 条
[1]   A ROLE FOR HSP90 IN CELL-CYCLE CONTROL - WEE1 TYROSINE KINASE-ACTIVITY REQUIRES INTERACTION WITH HSP90 [J].
ALIGUE, R ;
AKHAVANNIAK, H ;
RUSSELL, P .
EMBO JOURNAL, 1994, 13 (24) :6099-6106
[2]   MICROFILAMENTS, CELL-SHAPE CHANGES, AND THE FORMATION OF PRIMARY MESENCHYME IN SEA-URCHIN EMBRYOS [J].
ANSTROM, JA .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1992, 264 (03) :312-322
[3]   DEVELOPMENT OF MYOPLASM-ENRICHED ASCIDIAN EMBRYOS [J].
BATES, WR .
DEVELOPMENTAL BIOLOGY, 1988, 129 (01) :241-252
[4]   ALKALINE-PHOSPHATASE EXPRESSION IN ASCIDIAN EGG FRAGMENTS AND ANDROMEROGONS [J].
BATES, WR ;
JEFFERY, WR .
DEVELOPMENTAL BIOLOGY, 1987, 119 (02) :382-389
[5]   TRANSLATIONAL ACTIVATION OF MATERNAL MESSENGER-RNA ENCODING THE HEAT-SHOCK PROTEIN HSP90 DURING SEA-URCHIN EMBRYOGENESIS [J].
BEDARD, PA ;
BRANDHORST, BP .
DEVELOPMENTAL BIOLOGY, 1986, 117 (01) :286-293
[6]   Ubiquitination of neuronal nitric-oxide synthase in vitro and in vivo [J].
Bender, AT ;
Demady, DR ;
Osawa, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17407-17411
[7]   Neuronal nitric-oxide synthase is regulated by the hsp90-based chaperone system in vivo [J].
Bender, AT ;
Silverstein, AM ;
Demady, DR ;
Kanelakis, KC ;
Noguchi, S ;
Pratt, WB ;
Osawa, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (03) :1472-1478
[8]   ROLE OF THE EXTRACELLULAR-MATRIX IN TISSUE-SPECIFIC GENE-EXPRESSION IN THE SEA-URCHIN EMBRYO [J].
BENSON, S ;
RAWSON, R ;
KILLIAN, C ;
WILT, F .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1991, 29 (03) :220-226
[9]   Regulation of metamorphosis in ascidians involves NO/cGMP signaling and HSP90 [J].
Bishop, CD ;
Bates, WR ;
Brandhorst, BP .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 2001, 289 (06) :374-384
[10]  
BISHOP CD, 2001, BIOL BULL, P394