Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

被引：41

作者：

Rodriguez-Lopez, Pedro ^{[1
,2
]}

Carballo-Justo, Alba ^{[1
]}

Draper, Lorraine A. ^{[3
]}

Cabo, Marta L. ^{[1
]}

机构：

[1] CSIC, IIM, Dept Microbiol & Technol Marine Prod, Pontevedra, Spain

[2] Autonomous Univ Barcelona, Dept Genet & Microbiol, Fac Biosci, Catalonia, Spain

[3] Univ Coll Cork, APC Microbiome Inst, Cork, Ireland

来源：

BIOFOULING | 2017年 / 33卷 / 01期

关键词：

Benzalkonium chloride; biofilm; disinfection; enzymes; fluorescence microscopy; Listeria monocytogenes; FOOD-INDUSTRY; EXTRACELLULAR DNA; STAINLESS-STEEL; IN-VITRO; STAPHYLOCOCCUS-EPIDERMIDIS; BACTERIAL BIOFILMS; CURRENT KNOWLEDGE; FORM BIOFILMS; RESISTANCE; PATHOGENS;

D O I：

10.1080/08927014.2016.1261847

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes-Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI-BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI-BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control.

引用

页码：45 / 58

页数：14

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