Spectrophotometric determination of ciprofloxacin, enrofloxacin and pefloxacin through charge transfer complex formation

A spectrophotometric method was described for the determination of the antibacterial quinolone derivatives, ciprofloxacin, enrofloxacin and pefloxacin through charge transfer complex formation with three different acceptors. Chloranilic acid (CL) was utilized for their determination, forming charge transfer complex with lambda max 520 nm. The proposed method was applied for determination of Ciprocin tablets, Enroxil oral solution, Peflacin ampoules and Peflacin tablets, with mean percentage accuracies, 99.58 +/-1.25, 99.94 +/-0.96, 100.91 +/-1.59 and 99.86 +/-1.003. Also, tetracyanoethylene (TONE) was utilized in the determination of the concerned compounds forming charge transfer complexes with maximum absorbances at lambda max 335 nm for ciprofloxacin and at lambda max 290 nm for both enrofloxacin and pefloxacin. The procedure was applied for determination of Ciprocin tablets, Enroxil 10% oral solution, Peflacine tablets and Peflacine ampoules with mean percentage accuracies 99.40 +/-1.27, 99.95 +/-0.90, 98.98 +/-1.565 and 99.88 +/-0.998, respectively. Also, 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) was utilized for determination of pefloxacin forming charge transfer complex with maximum absorbance at lambda max 460 nm. The procedure was applied for determination of peflacine tablets and peflacine ampoules with mean percentage accuracies 100.40 +/-0.76 and 99.91 +/-0.623, respectively. Statistical analysis of the obtained results showed no significant difference between the proposed method and other official and reported methods as evident from the t-test and variance ratio. (C) 2002 Elsevier Science B.V. All rights reserved.