Genetic diversity of native bradyrhizobia isolated from soybeans (Glycine max L.) in different agricultural-ecological-climatic regions of India

被引：70

作者：

Appunu, Chinnaswamy ^{[1
,2
,3
]}

N'Zoue, Angele ^{[4
]}

Laguerre, Gisele ^{[2
,3
,4
]}

机构：

[1] Banaras Hindu Univ, Inst Agr Sci, Dept Genet & Plant Breeding, Microbial Genet Lab, Varanasi 221005, Uttar Pradesh, India

[2] INRA, UMR Microbiol & Geochim Sols 1229, F-21065 Dijon, France

[3] Univ Bourgogne, UMR 1229, F-21065 Dijon, France

[4] IRD, UMR Symbioses Trop & Mediterraneennes 113, F-34398 Montpellier, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 19期

关键词：

D O I：

10.1128/AEM.01320-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fifty isolates from root nodules of soybean plants sampled in five agricultural-ecological-climatic regions of India were analyzed by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene, the intergenic spacer region between the 16S and 23S rRNA genes (IGS), and the nifH and nodC genes. Eight haplotypes assigned to the Bradyrhizobium genus were identified, and the genetic diversity was conserved across regions. Sequence analyses of the IGS and the dnaK, glnII, recA, and nifH genes revealed three groups. One of them (26% of isolates) was assigned to Bradyrhizobium liaoningense. A second group (36% of isolates) was identified as B. yuanmingense but likely forms a new biovar able to nodulate soybean plants. The third lineage (38% of isolates) was different from all described Bradyrhizobium species but showed the same symbiotic genotype as B. liaoningense and B. japonicum bv. glycinearum.

引用

页码：5991 / 5996

页数：6

共 33 条

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