The sortase SrtA of Listeria monocytogenes is involved in processing of internalin and in virulence

被引:101
作者
Garandeau, C [1 ]
Réglier-Poupet, H [1 ]
Dubail, L [1 ]
Beretti, JL [1 ]
Berche, P [1 ]
Charbit, A [1 ]
机构
[1] CHU Necker Enfants Malad, INSERM, U411, F-75730 Paris 15, France
关键词
D O I
10.1128/IAI.70.3.1382-1390.2002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Listeria monocytogenes is an intracellular gram-positive human pathogen that invades eucaryotic cells. Among the surface-exposed proteins playing a role in this invasive process, internalin belongs to the family of LPXTG proteins, which are known to be covalently linked to the bacterial cell wall in gram-positive bacteria. Recently, it has been shown in Staphylococcus aureus that the covalent anchoring of protein A, a typical LPXTG protein, is due to a cysteine protease, named sortase, required for bacterial virulence. Here, we identified in silico from the genome of L. monocytogenes a gene, designated srtA, encoding a sortase homologue. The role of this previously unknown sortase was studied by constructing a sortase knockout mutant. Internalin was used as a reporter protein to study the effects of the srtA mutation on cell wall anchoring of this LPXTG protein in L. monocytogenes. We show that the srtA mutant (i) is affected in the display of internalin at the bacterial surface, (if) is significantly less invasive in vitro, and (iii) is attenuated in its virulence in the mouse. These results demonstrate that srtA of L. monocytogenes acts as a sortase and plays a role in the pathogenicity.
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页码:1382 / 1390
页数:9
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