Growth energetics of an alkaline serine protease-producing strain of Bacillus clausii during continuous cultivation

被引:8
作者
Christiansen, T
Nielsen, J
机构
[1] Tech Univ Denmark, Bioctr DTU, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark
[2] Novozymes AS, DK-2880 Bagsvaerd, Denmark
关键词
D O I
10.1007/s00449-001-0276-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Glucose-limited chemostats were used to determine the growth yields of biomass of Bacillus clausii PP 473-8 producing an alkaline serine protease Savinase (Novozymes A/S, Bagsvaerd, Denmark) and a low yield of biomass on oxygen was observed. The energy metabolism was investigated further by setting up simple stoichiometric models for growth on glucose and citrate. In order to determine the parameters in the models, a macromolecular biomass composition was determined based on measured values of protein and RNA combined with literature data. From the macromolecular composition of the biomass the theoretical co-factor and building block requirements needed for biomass formation were calculated. Using the stoichiometric models and data for growth on glucose and citrate the amount of ATP needed for biomass synthesis was estimated to 42.0 mmol ATP/gDW, the P/O ratio to 0.68 and the ATP maintenance to 2.93 mmol ATP/gDW/h. From these values it is concluded that the high oxygen consumption compared with other Bacillus species is due to a low efficiency in respiration resulting in a low P/O ratio. Finally, the energetic parameters were estimated for different architectures of the respiratory chain.
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收藏
页码:329 / 339
页数:11
相关论文
共 44 条
[1]  
AONO R, 1983, J GEN MICROBIOL, V129, P1083
[2]   COMPOSITION OF THE PEPTIDOGLYCAN OF ALKALOPHILIC BACILLUS SPP [J].
AONO, R ;
HORIKOSHI, K ;
GOTO, S .
JOURNAL OF BACTERIOLOGY, 1984, 157 (02) :688-689
[3]  
AONO R, 1989, J GEN MICROBIOL, V135, P265
[4]  
Arbige M. V., 1993, P871
[5]  
Archibald A. R., 1993, P381
[6]   AMINO-ACID ANALYSIS - DETERMINATION OF CYSTEINE PLUS HALF-CYSTINE IN PROTEINS AFTER HYDROCHLORIC-ACID HYDROLYSIS WITH A DISULFIDE COMPOUND AS ADDITIVE [J].
BARKHOLT, V ;
JENSEN, AL .
ANALYTICAL BIOCHEMISTRY, 1989, 177 (02) :318-322
[7]   GROWTH ENERGETICS OF LACTOCOCCUS-CREMORIS FD1 DURING ENERGY-LIMITATION, CARBON-LIMITATION, AND NITROGEN-LIMITATION IN STEADY-STATE AND TRANSIENT CULTURES [J].
BENTHIN, S ;
SCHULZE, U ;
NIELSEN, J ;
VILLADSEN, J .
CHEMICAL ENGINEERING SCIENCE, 1994, 49 (05) :589-609
[8]   A SIMPLE AND RELIABLE METHOD FOR THE DETERMINATION OF CELLULAR RNA-CONTENT [J].
BENTHIN, S ;
NIELSEN, J ;
VILLADSEN, J .
BIOTECHNOLOGY TECHNIQUES, 1991, 5 (01) :39-42
[9]   CHEMICAL COMPOSITION OF CYTOPLASMIC MEMBRANE OF BACILLUS SUBTILIS [J].
BISHOP, DG ;
RUTBERG, L ;
SAMUELSSON, B .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1967, 2 (04) :448-+
[10]   Secondary transporters for citrate and the Mg2+-citrate complex in Bacillus subtilis are homologous proteins [J].
Boorsma, A ;
vanderRest, ME ;
Lolkema, JS ;
Konings, WN .
JOURNAL OF BACTERIOLOGY, 1996, 178 (21) :6216-6222