Ghrelin receptors in rat and human nodose ganglia: putative role in regulating CB-1 and MCH receptor abundance

被引:145
作者
Burdyga, Galina
Varro, Andrea
Dimaline, Rod
Thompson, David G.
Dockray, Graham J.
机构
[1] Univ Liverpool, Sch Biomed Sci, Physiol Lab, Liverpool L69 3BX, Merseyside, England
[2] Univ Manchester, Hope Hosp, Dept Gastroenterol, Manchester M13 9PL, Lancs, England
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2006年 / 290卷 / 06期
基金
英国医学研究理事会;
关键词
vagus nerve; satiety; appetite; melanin-concentrating hormone; cholecystokinin; cannabinoid;
D O I
10.1152/ajpgi.00543.2005
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Intact vagal afferent neurons are required for the satiety effects of the intestinal hormone cholecystokinin (CCK) and the orexigenic effects of the gastric regulatory peptide ghrelin. In this study, we examined the localization of ghrelin receptors in nodose ganglia and their function in regulating the expression of other orexigenic receptors, notably cannabinoid (CB)-1 and melanin-concentrating hormone (MCH)-1 receptors. With the use of RTPCR, transcripts corresponding to both functional [ growth hormone secretagogue receptor (GHS-R)1a] and truncated forms (GHS-R1b) of the ghrelin receptor were detected in rat nodose ganglia. There was no difference in expression between rats fed ad libitum or fasted for up to 48 h. Immunohistochemical studies using antibodies directed at GHS-R1a revealed expression in over 75% of neurons also expressing CCK-1 receptors in the mid- and caudal regions of the ganglion. There was also expression in human nodose ganglia. In fasted rats in which CB-1 and MCH-1 receptor expression was increased, administration of ghrelin prevented the downregulation by refeeding. We conclude that the actions of CCK and ghrelin are mediated by a common population of vagal afferent neurons. Ghrelin may act to limit the action of CCK in depressing expression of CB-1 and MCH-1 receptors and other receptors.
引用
收藏
页码:G1289 / G1297
页数:9
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