Thromboxane A(2) stimulates mitogen-activated protein kinase and arachidonic acid liberation in rabbit platelets

被引：28

作者：

Ohkubo, S ^{[1
]}

Nakahata, N ^{[1
]}

Ohizumi, Y ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT MOL BIOL,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

PROSTAGLANDINS | 1996年 / 52卷 / 05期

关键词：

thromboxane A(2); tyrosine kinase; mitogen-activated protein kinase; arachidonic acid; cytosolic phospholipase A(2);

D O I：

10.1016/S0090-6980(96)00105-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

U46619, a thromboxane A(2) mimetic, caused tyrosine phosphorylation of several proteins in rabbit platelets. Among them, 42 kDa protein was identified as a mitogen-activated protein kinase (MAPK). U46619 activated MAPK in a concentration-dependent manner, measured by incorporation of P-32 to a specific substrate for MAPK. U46619 also liberated [H-3]arachidonic acid in a concentration-dependent manner. The U46619-induced MAPK activation and [H-3]arachidonic acid liberation were inhibited by SQ29548 and by the removal of external Ca2+ ions. This is a first demonstration that TXA(2) activates MAPK accompanied with arachidonic acid liberation in rabbit platelets.

引用

页码：403 / 413

页数：11

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