Visualizing one-dimensional diffusion of proteins along DNA

被引：229

作者：

Gorman, Jason ^{[2
]}

Greene, Eric C. ^{[1
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[2] Columbia Univ, Dept Biol Sci, New York, NY 10032 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2008年 / 15卷 / 08期

关键词：

D O I：

10.1038/nsmb.1441

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of proteins to locate specific target sequences or structures among a vast excess of nonspecific DNA is a fundamental property that affects virtually all aspects of biology. Despite this importance, experimental methods have lagged behind the establishment of theoretical principles describing potential target location mechanisms. However, recent advances in single-molecule detection now allow direct visual observation of proteins diffusing along DNA. Here we present an overview of these new observations and discuss the advantages, limitations and future prospects for imaging the motion of proteins along DNA.

引用

页码：768 / 774

页数：7

共 62 条

[1] The coordinated functions of the E-coli MutS and MutL proteins in mismatch repair [J].

Acharya, S ;

Foster, PL ;

Brooks, P ;

Fishel, R .

MOLECULAR CELL, 2003, 12 (01) :233-246

[2]

Adam G., 1968, Struct. Chem. Mol. Biol, P198

[3] Diffusion constant of a nonspecifically bound protein undergoing curvilinear motion along DNA [J].

Bagchi, Biman ;

Blainey, Paul C. ;

Xie, X. Sunney .

JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (19) :6282-6284

[4] DIFFUSION-DRIVEN MECHANISMS OF PROTEIN TRANSLOCATION ON NUCLEIC-ACIDS .1. MODELS AND THEORY [J].

BERG, OG ;

WINTER, RB ;

VONHIPPEL, PH .

BIOCHEMISTRY, 1981, 20 (24) :6929-6948

[5] ASSOCIATION KINETICS WITH COUPLED 3-DIMENSIONAL AND ONE-DIMENSIONAL DIFFUSION - CHAIN-LENGTH DEPENDENCE OF THE ASSOCIATION RATE TO SPECIFIC DNA SITES [J].

BERG, OG ;

EHRENBERG, M .

BIOPHYSICAL CHEMISTRY, 1982, 15 (01) :41-51

[6] A base-excision DNA-repair protein finds intrahelical lesion bases by fast sliding in contact with DNA [J].

Blainey, PC ;

van Oijent, AM ;

Banerjee, A ;

Verdine, GL ;

Xie, XS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (15) :5752-5757

[7] Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA [J].

Bonnet, Isabelle ;

Biebricher, Andreas ;

Porte, Pierre-Louis ;

Loverdo, Claude ;

Benichou, Olivier ;

Voituriez, Raphael ;

Escude, Christophe ;

Wende, Wolfgang ;

Pingoud, Alfred ;

Desbiolles, Pierre .

NUCLEIC ACIDS RESEARCH, 2008, 36 (12) :4118-4127

[8] Facilitated target location on DNA by individual Escherichia coli RNA polymerase molecules observed with the scanning force microscope operating in liquid [J].

Bustamante, C ;

Guthold, M ;

Zhu, XS ;

Yang, GL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (24) :16665-16668

[9] Chromatin remodeling: insights and intrigue from single-molecule studies [J].

Cairns, Bradley R. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (11) :989-996

[10]

DOWD DR, 1990, J BIOL CHEM, V265, P3424

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