Monitoring of real-time streptavidin-biotin binding kinetics using droplet microfluidics

被引：126

作者：

Srisa-Art, Monpichar ^{[1
]}

Dyson, Emily C. ^{[1
]}

deMello, Andrew J. ^{[1
]}

Edel, Joshua B. ^{[1
,2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 18期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1021/ac801199k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Rapid kinetic measurements are important in understanding chemical interactions especially for biological molecules. Herein, we present a droplet-based microfluidic platform to study streptavidin-biotin binding kinetics with millisecond time resolution. With integration of a confocal fluorescence detection system, individual droplets can be monitored and characterized online to extract kinetic information. Using this approach, binding kinetics between streptavidin and biotin were observed via fluorescence resonance energy transfer. The binding rate constant of streptavidin and biotin was found to be in a range of 3.0 x 10(6)-4.5 x 10(7) M-1 S-1.

引用

页码：7063 / 7067

页数：5

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