CUL3 and NRF2 Mutations Confer an NRF2 Activation Phenotype in a Sporadic Form of Papillary Renal Cell Carcinoma

被引：121

作者：

Ooi, Aikseng ^{[1
]}

Dykema, Karl ^{[1
]}

Ansari, Asif ^{[2
]}

Petillo, David ^{[1
]}

Snider, John ^{[1
]}

Kahnoski, Richard ^{[2
]}

Anema, John ^{[2
]}

Craig, David ^{[3
]}

Carpten, John ^{[3
]}

Teh, Bin-Tean ^{[4
,5
,6
]}

Furge, Kyle A. ^{[1
]}

机构：

[1] Van Andel Res Inst, Interdisciplinary Renal Oncol Lab, Grand Rapids, MI 49080 USA

[2] Spectrum Hlth, Grand Rapids, MI USA

[3] Translat Genom Res Inst, Phoenix, AZ USA

[4] Natl Canc Ctr Singapore, NCCS VARI Translat Canc Res Lab, Singapore, Singapore

[5] Singapore Gen Hosp, Singhlth, Singapore, Singapore

[6] Duke NUS, Singapore, Singapore

来源：

CANCER RESEARCH | 2013年 / 73卷 / 07期

关键词：

EXPRESSION ANALYSIS; OXIDATIVE STRESS; POINT MUTATIONS; BREAST-CANCER; KEAP1; INDUCTION; PATHWAY; INACTIVATION; SUCCINATION; FUMARATE;

D O I：

10.1158/0008-5472.CAN-12-3227

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Sustained activation of the stress-regulated transcription factor NRF2 (NFE2L2) is a prominent feature of many types of cancer, implying that mutations driving NRF2 may be important to tumor progression. In hereditary type 2 papillary renal cell carcinoma (PRCC2, also known as hereditary leiomyomatosis and renal cell cancer), NRF2 activation is a direct consequence of the accumulation of intracellular fumarate, a result of fumarate hydratase (FH) inactivation, but it is not clear how NRF2 may be activated in sporadic forms of PRCC2. Here we show that somatic mutations in NRF2, CUL3, and SIRT1 are responsible for driving the NRF2 activation phenotype in sporadic PRCC2. Transcriptome sequencing revealed the expression pattern of mutant alleles of NRF2, CUL3, and SIRT1 and also confirmed NRF2 activation in clinical specimens. Our results show a convergence in somatic mutations in sporadic PRCC2 with FH mutation in hereditary PRCC2. Cancer Res; 73(7); 2044-51. (C)2013 AACR.

引用

页码：2044 / 2051

页数：8

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