Middle East respiratory syndrome coronavirus (MERS-CoV) causes transient lower respiratory tract infection in rhesus macaques

被引：245

作者：

de Wit, Emmie ^{[1
]}

Rasmussen, Angela L. ^{[4
]}

Falzarano, Darryl ^{[1
]}

Bushmaker, Trenton ^{[1
]}

Feldmann, Friederike ^{[2
]}

Brining, Douglas L. ^{[2
]}

Fischer, Elizabeth R. ^{[3
]}

Martellaro, Cynthia ^{[1
]}

Okumura, Atsushi ^{[4
]}

Chang, Jean ^{[4
]}

Scott, Dana ^{[2
]}

Benecke, Arndt G. ^{[4
,5
]}

Katze, Michael G. ^{[4
]}

Feldmann, Heinz ^{[1
,6
]}

Munster, Vincent J. ^{[1
]}

机构：

[1] NIAID, Lab Virol, NIH, Hamilton, MT 59840 USA

[2] NIAID, Rocky Mt Vet Branch, NIH, Hamilton, MT 59840 USA

[3] NIAID, Microscopy Unit, Res Technol Branch, Div Intramural Res,NIH, Hamilton, MT 59840 USA

[4] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[5] Univ Paris 06, Dept Biol, Ctr Natl Rech Sci, Unite Mixte Rech 7224, F-75005 Paris, France

[6] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB R3E 0J9, Canada

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 41期

基金：

美国国家卫生研究院;

关键词：

emerging infectious disease; DPP4; CLINICAL-FEATURES; AFRICAN-GREEN; ANIMAL-MODEL; VIRUS; PNEUMONIA; SYSTEM; BATS;

D O I：

10.1073/pnas.1310744110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In 2012, a novel betacoronavirus, designated Middle East respiratory syndrome coronavirus or MERS-CoV and associated with severe respiratory disease in humans, emerged in the Arabian Peninsula. To date, 108 human cases have been reported, including cases of human-to-human transmission. The availability of an animal disease model is essential for understanding pathogenesis and developing effective countermeasures. Upon a combination of intratracheal, ocular, oral, and intranasal inoculation with 7 x 10(6) 50% tissue culture infectious dose of the MERS-CoV isolate HCoV-EMC/2012, rhesus macaques developed a transient lower respiratory tract infection. Clinical signs, virus shedding, virus replication in respiratory tissues, gene expression, and cytokine and chemokine profiles peaked early in infection and decreased over time. MERS-CoV caused a multifocal, mild to marked interstitial pneumonia, with virus replication occurring mainly in alveolar pneumocytes. This tropism of MERS-CoV for the lower respiratory tract may explain the severity of the disease observed in humans and the, up to now, limited human-to-human transmission.

引用

页码：16598 / 16603

页数：6

共 30 条

[1] Human Betacoronavirus 2c EMC/2012-related Viruses in Bats, Ghana and Europe [J].

Annan, Augustina ;

Baldwin, Heather J. ;

Corman, Victor Max ;

Klose, Stefan M. ;

Owusu, Michael ;

Nkrumah, Evans Ewald ;

Badu, Ebenezer Kofi ;

Anti, Priscilla ;

Agbenyega, Olivia ;

Meyer, Benjamin ;

Oppong, Samuel ;

Sarkodie, Yaw Adu ;

Kalko, Elisabeth K. V. ;

Lina, Peter H. C. ;

Godlevska, Elena V. ;

Reusken, Chantal ;

Seebens, Antje ;

Gloza-Rausch, Florian ;

Vallo, Peter ;

Tschapka, Marco ;

Drosten, Christian ;

Drexler, Jan Felix .

EMERGING INFECTIOUS DISEASES, 2013, 19 (03) :456-459

[2] Coronaviruses in bats from Mexico [J].

Anthony, S. J. ;

Ojeda-Flores, R. ;

Rico-Chavez, O. ;

Navarrete-Macias, I. ;

Zambrana-Torrelio, C. M. ;

Rostal, M. K. ;

Epstein, J. H. ;

Tipps, T. ;

Liang, E. ;

Sanchez-Leon, M. ;

Sotomayor-Bonilla, J. ;

Aguirre, A. A. ;

Avila-Flores, R. ;

Medellin, R. A. ;

Goldstein, T. ;

Suzan, G. ;

Daszak, P. ;

Lipkin, W. I. .

JOURNAL OF GENERAL VIROLOGY, 2013, 94 :1028-1038

[3] Hospital Outbreak of Middle East Respiratory Syndrome Coronavirus [J].

Assiri, Abdullah ;

McGeer, Allison ;

Perl, Trish M. ;

Price, Connie S. ;

Al Rabeeah, Abdullah A. ;

Cummings, Derek A. T. ;

Alabdullatif, Zaki N. ;

Assad, Maher ;

Almulhim, Abdulmohsen ;

Makhdoom, Hatem ;

Madani, Hossam ;

Alhakeem, Rafat ;

Al-Tawfiq, Jaffar A. ;

Cotten, Matthew ;

Watson, Simon J. ;

Kellam, Paul ;

Zumla, Alimuddin I. ;

Memish, Ziad A. .

NEW ENGLAND JOURNAL OF MEDICINE, 2013, 369 (05) :407-416

[4] Tropism of and Innate Immune Responses to the Novel Human Betacoronavirus Lineage C Virus in Human Ex Vivo Respiratory Organ Cultures [J].

Chan, Renee W. Y. ;

Chan, Michael C. W. ;

Agnihothram, Sudhakar ;

Chan, Louisa L. Y. ;

Kuok, Denise I. T. ;

Fong, Joanne H. M. ;

Guan, Y. ;

Poon, Leo L. M. ;

Baric, Ralph S. ;

Nicholls, John M. ;

Peiris, J. S. Malik .

JOURNAL OF VIROLOGY, 2013, 87 (12) :6604-6614

[5]

Corman VM, 2012, EUROSURVEILLANCE, V17, P3

[6] Middle East Respiratory Syndrome Coronavirus (MERS-CoV): Announcement of the Coronavirus Study Group [J].

de Groot, Raoul J. ;

Baker, Susan C. ;

Baric, Ralph S. ;

Brown, Caroline S. ;

Drosten, Christian ;

Enjuanes, Luis ;

Fouchier, Ron A. M. ;

Galiano, Monica ;

Gorbalenya, Alexander E. ;

Memish, Ziad A. ;

Perlman, Stanley ;

Poon, Leo L. M. ;

Snijder, Eric J. ;

Stephens, Gwen M. ;

Woo, Patrick C. Y. ;

Zaki, Ali M. ;

Zambon, Maria ;

Ziebuhr, John .

JOURNAL OF VIROLOGY, 2013, 87 (14) :7790-7792

[7] Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection [J].

Drosten, Christian ;

Seilmaier, Michael ;

Corman, Victor M. ;

Hartmann, Wulf ;

Scheible, Gregor ;

Sack, Stefan ;

Guggemos, Wolfgang ;

Kallies, Rene ;

Muth, Doreen ;

Junglen, Sandra ;

Muller, Marcel A. ;

Haas, Walter ;

Guberina, Nana ;

Rohnisch, Tim ;

Schmid-Wendtner, Monika ;

Aldabbagh, Souhaib ;

Dittmer, Ulf ;

Gold, Hermann ;

Graf, Petra ;

Bonin, Frank ;

Rambaut, Andrew ;

Wendtner, Clemens-Martin .

LANCET INFECTIOUS DISEASES, 2013, 13 (09) :745-751

[8] Aetiology -: Koch's postulates fulfilled for SARS virus [J].

Fouchier, RAM ;

Kuiken, T ;

Schutten, M ;

van Amerongen, G ;

van Doornum, J ;

van den Hoogen, BG ;

Peiris, M ;

Lim, W ;

Stöhr, K ;

Osterhaus, ADME .

NATURE, 2003, 423 (6937) :240-240

[9] Pneumonitis and multi-organ system disease in common marmosets (Callithrix jacchus) infected with the severe acute respiratory syndrome-associated coronavirus [J].

Greenough, TC ;

Carville, A ;

Coderre, J ;

Somasundaran, M ;

Sullivan, JL ;

Luzuriaga, K ;

Mansfield, K .

AMERICAN JOURNAL OF PATHOLOGY, 2005, 167 (02) :455-463

[10] Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission [J].

Guery, Benoit ;

Poissy, Julien ;

el Mansouf, Loubna ;

Sejourne, Caroline ;

Ettahar, Nicolas ;

Lemaire, Xavier ;

Vuotto, Fanny ;

Goffard, Anne ;

Behillil, Sylvie ;

Enouf, Vincent ;

Caro, Valerie ;

Mailles, Alexandra ;

Che, Didier ;

Manuguerra, Jean-Claude ;

Mathieu, Daniel ;

Fontanet, Arnaud ;

van der Werf, Sylvie .

LANCET, 2013, 381 (9885) :2265-2272

← 1 2 3 →