Immobilization of Rhodococcus sp AJ270 in alginate capsules and its application in enantio selective biotransformation of trans-2-methyl-3-phenyl-oxiranecarbonitrile and amide

被引：22

作者：

Guo, Xing-Lin

Deng, Gang

Xu, Jian

Wang, Mei-Xiang ^{[2
]}

机构：

[1] Chinese Acad Sci, State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China

[2] Chinese Acad Sci, Biol Chem Lab, Inst Chem, Beijing 100080, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 01期

基金：

中国国家自然科学基金;

关键词：

encapsulation; Rhodococcus sp AJ270; enantioselective biotransformation; nitrile hydratase; amidase;

D O I：

10.1016/j.enzmictec.2005.01.044

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rhodococcus sp. AJ270, a nitrile hydratase/amidase-containing microorganism, were immobilized in alginate capsules. The influences of the bead size and the crosslinking agent on morphology and the biocatalytic activity of the cells were examined. In the presence of an organic co-solvent, such as methanol or acetone (5%), the encapsulated Rhodococcus sp. AJ270 has been found to catalyze efficiently the biotransformation of racemic trans-2-methyl-3-phenyl-oxiranecarbonitrile and its amide to yield highly enantiopure 2R,3S-(-)-2-methyl-3-phenyl-oxiranecarboxamide in at least seven runs. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1 / 5

页数：5

共 37 条

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