Method for quantification of aerobic anoxygenic phototrophic bacteria

被引：14

作者：

Zhang, Y ^{[1
]}

Jiao, NZ ^{[1
]}

机构：

[1] Xiamen Univ, Minist Educ, Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2004年 / 49卷 / 06期

关键词：

AAPB; EFM-IRP; quantification; cyanobacteria;

D O I：

10.1360/03wc0447

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Accurate quantification of aerobic anoxygenic phototrophic bacteria (AAPB) is of crucial importance for estimation of the role of AAPB in the carbon cycling in marine ecosystems. The normally used method "epifluorescence microscope-infrared photography (EFM-IRP)" is, however, subject to positive errors introduced by mistaking cyanobacteria as AAPB due to the visibility of cyanobacteria under infrared photographic conditions for AAPB. This error could be up to 30% in the coast of the East China Sea. Such bias should be avoided by either subtracting cyanobacteira from the total infrared counts or using a flowcytometer equipped with specific detectors for discrimination between cyanobacteria and AAPB.

引用

页码：597 / 599

页数：3

共 13 条

[1]

On the natural selection and evolution of the aerobic phototrophic bacteria [J].