Production of transgenic maize plants and progeny by bombardment of Hi-II immature embryos

被引：7

作者：

Songstad, DD ^{[1
]}

Armstrong, CL ^{[1
]}

Petersen, WL ^{[1
]}

Hairston, B ^{[1
]}

Hinchee, MAW ^{[1
]}

机构：

[1] MONSANTO CO,ST LOUIS,MO 63198

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 1996年 / 32卷 / 03期

关键词：

transformation; Zea mays L; histology; progeny;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Production of transgenic maize (Zea mays L.) callus, plants, and progeny from microprojectile bombardment of 2-5-d cultured Hi-II immature embryos is described. Histological evidence indicates that these tissues are amenable to transformation due to surface layer cell division of the scutellum. Two out of every 100 bombarded embryos produced transgenic callus and R(0) transgenic plants were both male and female fertile. Expected segregation of transgenes was observed in progeny. The primary advantage of bombarding these tissues is increased male and female fertility of transgenic plants compared with those produced using long-term callus or suspension cultures.

引用

页码：179 / 183

页数：5

共 30 条

[1]

Armstrong C. L., 1991, Maize Genetics Cooperation News Letter, P92

[2] ESTABLISHMENT AND MAINTENANCE OF FRIABLE, EMBRYOGENIC MAIZE CALLUS AND THE INVOLVEMENT OF L-PROLINE [J].