The human papillomavirus type 16 (HPV-16) E5 protein sensitizes human keratinocytes to apoptosis induced by osmotic stress

被引:23
作者
Kabsch, K [1 ]
Alonso, A [1 ]
机构
[1] Deutsch Krebsforschungszentrum, D-69120 Heidelberg, Germany
关键词
fpapillomavirus; keratinocytes; apoptosis; osmotic shock;
D O I
10.1038/sj.onc.1205147
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have studied the role of the HPV-16 E5 protein in apoptosis, using HaCaT cell lines stably transfected with either E5 (HaCaT/E5) or the empty vector (HaCaT/ pMSG) as control. When subjected to a hyperosmolar concentration of sorbitol, HaCaT/E5 cells respond with cytochrome e release, activation of caspase-3, -8, and -9, and PARP-cleavage, showing that the mitochondria and death-receptor mediated apoptotic pathways are involved in subsequent cell death. Similar effects are observed for the control cells only after extended sorbitol treatment. Thus, E5-expressing cells are more sensitive to osmotic stress, perhaps because of modifications of the cellular membranes caused by this strongly hydrophobic molecule.
引用
收藏
页码:947 / 953
页数:7
相关论文
共 33 条
[1]   Ultraviolet light induces apoptosis via direct activation of CD95 (Fas/APO-1) independently of its ligand CD95L [J].
Aragane, Y ;
Kulms, D ;
Metze, D ;
Wilkes, G ;
Pöppelmann, B ;
Luger, TA ;
Schwarz, T .
JOURNAL OF CELL BIOLOGY, 1998, 140 (01) :171-182
[2]   p38 mitogen-activated protein kinase regulates a novel, caspase-independent pathway for the mitochondrial cytochrome c release in ultraviolet B radiation-induced apoptosis [J].
Assefa, Z ;
Vantieghem, A ;
Garmyn, M ;
Declercq, W ;
Vandenabeele, P ;
Vandenheede, JR ;
Bouillon, R ;
Merlevede, W ;
Agostinis, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (28) :21416-21421
[3]   NORMAL KERATINIZATION IN A SPONTANEOUSLY IMMORTALIZED ANEUPLOID HUMAN KERATINOCYTE CELL-LINE [J].
BOUKAMP, P ;
PETRUSSEVSKA, RT ;
BREITKREUTZ, D ;
HORNUNG, J ;
MARKHAM, A ;
FUSENIG, NE .
JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :761-771
[4]   Biochemical pathways of caspase activation during apoptosis [J].
Budihardjo, I ;
Oliver, H ;
Lutter, M ;
Luo, X ;
Wang, XD .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1999, 15 :269-290
[5]   THE HUMAN PAPILLOMAVIRUS TYPE-6 AND 16-E5 PROTEINS ARE MEMBRANE-ASSOCIATED PROTEINS WHICH ASSOCIATE WITH THE 16-KILODALTON PORE-FORMING PROTEIN [J].
CONRAD, M ;
BUBB, VJ ;
SCHLEGEL, R .
JOURNAL OF VIROLOGY, 1993, 67 (10) :6170-6178
[6]   The human papillomavirus type 16 E5 protein modulates phospholipase C-γ-1 activity and phosphatidyl inositol turnover in mouse fibroblasts [J].
Crusius, K ;
Kaszkin, M ;
Kinzel, V ;
Alonso, A .
ONCOGENE, 1999, 18 (48) :6714-6718
[7]   The human papillomavirus type 16 E5-protein modulates ligand-dependent activation of the EGF receptor family in the human epithelial cell line HaCaT [J].
Crusius, K ;
Auvinen, E ;
Steuer, B ;
Gaissert, H ;
Alonso, A .
EXPERIMENTAL CELL RESEARCH, 1998, 241 (01) :76-83
[8]   The human papillomavirus type 16 E5 protein modulates ERK1/2 and p38 MAP kinase activation by an EGFR-Independent process in stressed human keratinocytes [J].
Crusius, K ;
Rodriguez, I ;
Alonso, A .
VIRUS GENES, 2000, 20 (01) :65-69
[9]   Mammalian caspases: Structure, activation, substrates, and functions during apoptosis [J].
Earnshaw, WC ;
Martins, LM ;
Kaufmann, SH .
ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 :383-424
[10]  
FAULKNERVALLE G, 1995, J GEN VIROL, V76, P1239