In situ detection of calcium ions with chemically modified microcantilevers

被引:57
作者
Ji, HF [1 ]
Thundat, T [1 ]
机构
[1] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN 37831 USA
关键词
microcantilevers; calcium ion detection; selective coatings; self assembled monolayers;
D O I
10.1016/S0956-5663(01)00270-6
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We report a novel technique of micromechanical detection of trace amounts of calcium ions by using microcantilevers modified with ion-selective self-assembled monolayers (SAMs). The SAM-modified microcantilevers undergo bending due to selective adsorption of calcium ions. Experiments conducted under flow conditions show that the modified cantilevers respond sensitively to calcium ions (Ca2+); a Ca2+ concentration of 10(-9) M can be detected with this technique. Other cations, such as Na+ and K+, do not have any effect on the deflection of these cantilevers. We demonstrate two different kinds of SAMs having selectivity for calcium ions. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:337 / 343
页数:7
相关论文
共 27 条
  • [1] PREPARATION OF NEUTRAL IONOPHORES FOR ALKALI AND ALKALINE-EARTH METAL CATIONS AND THEIR APPLICATION IN ION-SELECTIVE MEMBRANE ELECTRODES
    AMMANN, D
    BISSIG, R
    GUGGI, M
    PRETSCH, E
    SIMON, W
    BOROWITZ, IJ
    WEISS, L
    [J]. HELVETICA CHIMICA ACTA, 1975, 58 (06) : 1535 - 1548
  • [2] AMMANN D, 1972, ANAL LETT, V5, P843
  • [3] AMMANN D, 1975, ANAL LETT, V8, P709
  • [4] BARNES JR, 1994, PHOTOTHERMAL SPECTRO, V372, P7981
  • [5] Surface stress in the self-assembly of alkanethiols on gold probed by a force microscopy technique
    Berger, R
    Delamarche, E
    Lang, HP
    Gerber, C
    Gimzewski, JK
    Meyer, E
    Guntherodt, HJ
    [J]. APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 1998, 66 (Suppl 1): : S55 - S59
  • [6] Surface stress in the self-assembly of alkanethiols on gold
    Berger, R
    Delamarche, E
    Lang, HP
    Gerber, C
    Gimzewski, JK
    Meyer, E
    Guntherodt, HJ
    [J]. SCIENCE, 1997, 276 (5321) : 2021 - 2024
  • [7] CALCIUM-SENSITIVE MICROELECTRODE SUITABLE FOR INTRACELLULAR MEASUREMENT OF CALCIUM(II) ACTIVITY
    BROWN, HM
    PEMBERTON, JP
    OWEN, JD
    [J]. ANALYTICA CHIMICA ACTA, 1976, 85 (02) : 261 - 276
  • [8] A sensitive method to measure changes in the surface stress of solids
    Butt, HJ
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 180 (01) : 251 - 260
  • [9] ADSORPTION-INDUCED SURFACE STRESS AND ITS EFFECTS ON RESONANCE FREQUENCY OF MICROCANTILEVERS
    CHEN, GY
    THUNDAT, T
    WACHTER, EA
    WARMACK, RJ
    [J]. JOURNAL OF APPLIED PHYSICS, 1995, 77 (08) : 3618 - 3622
  • [10] Sulfate ion sensing based on a quartz-crystal microbalance immobilized with barium sulfate crystals grown on a self-assembled monolayer of phosphorylated 11-mercapto-1-undecanol
    Eun, H
    Umezawa, Y
    [J]. ANALYTICA CHIMICA ACTA, 1998, 375 (1-2) : 155 - 165